SGER: BAC end Sequencing to Quality Control BAC Libraries

SGER：BAC 末端测序以进行质量控制 BAC 文库

• 批准号: 0306190
• 负责人: Norman Lee
• 金额: $ 7.38万
• 依托单位: Institute for Genomic Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-15 至 2005-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0306190&HistoricalAwards=false
• 关键词: SGER; BAC; end; Sequencing; Quality

0306190ZhaoDue to their large insert size and stability, Bacterial Artificial Chromosome (BAC) clones have emerged as an essential component in genome mapping, DNA sequencing, as well as comparative and functional genomics projects to answer evolutionary, agricultural and biomedical questions. As such, the National Science Foundation (NSF) has initiated a program that funds the construction of 31 animal BAC libraries and 32 plant BAC libraries from species spanning the phylogenetic tree of life. Clearly, these libraries should be of high quality to be most useful. Quality control therefore becomes an essential requirement. BAC end sequencing provides several pieces of critical information about a BAC library regarding contaminations, randomness and insert size. Here we propose to end sequence approximately 100 random clones from each library and conduct sequence analyses to assess the quality of the 63 BAC libraries that are being constructed under the NSF program.

细菌人工染色体(BAC)克隆由于其大的插入大小和稳定性，已成为基因组图谱、DNA测序以及比较和功能基因组学项目中的重要组成部分，以回答进化、农业和生物医学问题。因此，美国国家科学基金会(NSF)启动了一项计划，资助从生命系统发育树上的物种建立31个动物BAC文库和32个植物BAC文库。显然，这些库应该是高质量的，才是最有用的。因此，质量控制成为一项基本要求。BAC末端测序提供了几条关于BAC文库的关于污染、随机性和插入大小的关键信息。在这里，我们建议从每个文库中结束大约100个随机克隆的测序，并进行序列分析，以评估根据NSF计划正在构建的63个BAC文库的质量。