High density mapping of BAC end sequences onto medaka linkage groups

BAC 末端序列高密度映射到青鳉连锁群上

• 批准号: 11236210
• 负责人: TANAKA Minoru
• 金额: $ 125.7万
• 依托单位: Hokkaido University (2000-2003)Okazaki National Research Institutes (1999)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11236210/
• 关键词: medaka; genome; typing; STS marker; physical mapping; linkage; recombination; ゲノム; マッピング; BAC; 連鎖地図; EST; 生殖腺; 性; タイピング; 多型; マイクロサテライト; SSLP; 染色体地図; ゲノム解析; CAリピート; 染色体; 物理地図; トランジェニック; GFP

Physical mapping of medaka chromosomes was constructed based on the length polymorphism between two inbred strains, HNI and AA2. Fluorescent primers designed by BAC end sequences and nucleotide sequences having (CA) repeats were generated. 607 primer sets showing length polymorphism between the two strains were selected and were further used for typing to map onto the medaka linkage groups.More than 15% of the primers generated showed length polymorphism, confirming the high genomic polymorphism between the two strains.This information is the first systematic STS markers on medaka genomes and provides a powerful tool for anchoring BAC onto the linkage groups and for walk to the region of interest.Estimated physical length do not correspond to that estimated by anonymous markers such as RFLP and RAPD. Especially the length of linkage group 23 is estimated to be much longer than that estimated before. Regions of repression of recombination are different between female and male. It is inclined that recombination in male occurs more frequently at the distal regions of the linkages.

利用近交系HNI和AA2的染色体长度多态性，构建了medaka染色体物理图谱。以BAC端序列和具有（CA）重复序列的核苷酸序列设计荧光引物。选择607组长度多态性的引物，进行分型，定位medaka连锁群。超过15%的引物显示长度多态性，证实了两菌株之间的高度基因组多态性。这一信息是medaka基因组上第一个系统的STS标记，为将BAC锚定在连锁群上并行走到感兴趣的区域提供了有力的工具。估计的物理长度与匿名标记（如RFLP和RAPD）估计的物理长度不对应。特别是连锁基团23的长度估计比之前估计的要长得多。重组抑制区域在女性和男性之间是不同的。它倾向于重组在男性更频繁地发生在连接的远端区域。

项目成果

木下政人: "トランスジェニックフィッシュ系統の作出とプロモーター解析"蛋白質核酸酵素 増刊 小型魚類研究の新展開. 45. 2954-2961 (2000)

Masato Kinoshita：“转基因鱼系的创建和启动子分析”蛋白质核酸酶特刊小鱼研究新进展45。2954-2961（2000）。

Shibata, Y.: "Identification and cDNA cloning of alveolin, an extracellular metalloproteinase, which induces chorion hardening of medaka (Oryzias latipes) eggs upon fertilization."J.Biol.Chem.. 275. 8349-8354 (2000)

Shibata, Y.：“肺泡蛋白的鉴定和 cDNA 克隆，肺泡蛋白是一种细胞外金属蛋白酶，可在受精时诱导青鳉 (Oryzias latipes) 卵绒毛膜硬化。”J.Biol.Chem.. 275. 8349-8354 (2000)

田中実, 木下政人, 青木裕美子, 中村修平: "RNA翻訳制御による生殖細胞形成:メダカを中心に"細胞工学. 22(10). 1074-1076 (2003)

Minoru Tanaka、Masato Kinoshita、Yumiko Aoki、Shuhei Nakamura：“通过控制 RNA 翻译形成生殖细胞：聚焦青鳉”《细胞工程》22(10)。

田中実, 木下政人: "メダカの細胞1つ1つを光らせて生殖腺形成機構を解明する -生殖細胞系列特異的にRNA翻訳制御を指示するエレメントがわかった-"化学と生物. 41(5). 210-213 (2003)

Minoru Tanaka，Masato Kinoshita：“通过使每个青鳉细胞发光来阐明性腺形成的机制 - 指导种系特异性 RNA 翻译控制的元素 -” 化学与生物学 41(5) (2003)。

Tanaka, M.: "Teleost ovarian carbonyl reductase-like 20b-hydroxysteroid dehydrogenase : potential role in the production of maturation-inducing hormone during final oocyte maturation."Biol.Reprod.. 66. 1498-1504 (2002)

Tanaka, M.：“硬骨鱼卵巢羰基还原酶样 20b-羟基类固醇脱氢酶：最终卵母细胞成熟过程中成熟诱导激素产生的潜在作用。”Biol.Reprod.. 66. 1498-1504 (2002)