Analysis of phylogenetic relationships of Hordeum (Poaceae) polyploids

大麦（禾本科）多倍体系统发育关系分析

• 批准号: 162640843
• 负责人: Dr. Frank R. Blattner
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/162640843?language=en
• 关键词: Analysis; phylogenetic; relationships; Hordeum; Poaceae

Polyploidy, this means the duplication of entire genomes, is a very important mechanism creating biodiversity in plants. Within the barley genus Hordeum about half of the taxa are polyploids, mostly allopolyploids combining genomes from two or three different parental species. While for the diploid taxa a good hypothesis of phylogenetic relationships already exists, for polyploids few information is available. Knowledge of the phylogeny of a group is indispensable for further analyses of evolutionary or ecological processes. Within the proposed project I will analyze species relationships in Hordeum with three single copy nuclear marker loci by sequencing of cloned PCR products of these regions and using phylogenetic analysis algorithms. For some polyploid groups in-detail studies will be conducted including sequence analysis and genomic in situ hybridization (GISH). The work will result in a better understanding of this important grass genus, will establish new markers regions useful in grass or at least in Triticeae systematics, provide dating of polyploid origins, and will allow further analyses of genomic and ecological changes after or connected with polyploidization.

多倍体，这意味着整个基因组的复制是在植物中产生生物多样性的一种非常重要的机制。在大麦属中，大约一半的分类单元是多倍体，主要是将两个或三种不同亲本物种的基因组结合的同种倍体。对于二倍体类群，已经存在了系统发育关系的良好假设，但对于多倍体，很少有信息。对于进化或生态过程的进一步分析，对一组的系统发育的了解是必不可少的。在拟议的项目中，我将通过测序这些区域的克隆PCR产物并使用系统发育分析算法来分析霍尔德姆的物种关系，并通过三个单拷贝核标记基因座进行分析。对于某些多倍体组，将进行详细研究，包括序列分析和基因组原位杂交（GISH）。这项工作将使人们更好地了解这一重要的草属，建立新的标记区域对草或至少在Triticeae Systematics中有用，提供多倍体起源的日期，并允许在多倍体化或与多倍化后进行进一步分析基因组和生态变化。