Analysis of phylogenetic relationships of Hordeum (Poaceae) polyploids

大麦（禾本科）多倍体系统发育关系分析

• 批准号: 162640843
• 负责人: Dr. Frank R. Blattner
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/162640843?language=en
• 关键词: Analysis; phylogenetic; relationships; Hordeum; Poaceae

Polyploidy, this means the duplication of entire genomes, is a very important mechanism creating biodiversity in plants. Within the barley genus Hordeum about half of the taxa are polyploids, mostly allopolyploids combining genomes from two or three different parental species. While for the diploid taxa a good hypothesis of phylogenetic relationships already exists, for polyploids few information is available. Knowledge of the phylogeny of a group is indispensable for further analyses of evolutionary or ecological processes. Within the proposed project I will analyze species relationships in Hordeum with three single copy nuclear marker loci by sequencing of cloned PCR products of these regions and using phylogenetic analysis algorithms. For some polyploid groups in-detail studies will be conducted including sequence analysis and genomic in situ hybridization (GISH). The work will result in a better understanding of this important grass genus, will establish new markers regions useful in grass or at least in Triticeae systematics, provide dating of polyploid origins, and will allow further analyses of genomic and ecological changes after or connected with polyploidization.

多倍体，这意味着整个基因组的复制，是在植物中创造生物多样性的非常重要的机制。在大麦属中，大约一半的类群是多倍体，其中大部分是结合了来自两个或三个不同亲本物种的基因组的异源多倍体。虽然对于二倍体类群，系统发育关系的良好假设已经存在，但对于多倍体，可用的信息很少。了解一个群体的系统发育对于进一步分析进化或生态过程是必不可少的。在拟议的项目中，我将通过对这些区域的克隆 PCR 产物进行测序并使用系统发育分析算法，分析具有三个单拷贝核标记位点的大麦中的物种关系。对于一些多倍体群体，将进行详细研究，包括序列分析和基因组原位杂交（GISH）。这项工作将导致更好地了解这一重要的草属，将建立在草类或至少在小麦科系统学中有用的新标记区域，提供多倍体起源的测年，并将允许进一​​步分析多倍体化后或与之相关的基因组和生态变化。