Analysis of the phylogenetic relationships between the omon and Yayoi poputations through mitochondrial DNA sequence

通过线粒体DNA序列分析大蒙族和弥生族群的系统发育关系

基本信息

批准号：
11640709
负责人：
SHINODA Ken-ichi
金额：
$ 0.9万
依托单位：
佐賀医科大学
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11640709/
关键词：
mitochondria DNA D-loop region ancient DNA the origin of Japanese Jomon Yayoi Ancient DNA ミトコンドリア PCR法

项目摘要

Variation in the human mitochondrial genome (mtDNA) is now used to infer the histories of peoples, by means of the sequencing of parts of the D-loop region. We extracted DNA from the Jomon and Yayoi skeletal remains and, through PCR amplification, determined nucleotide sequences of their mtDNA D-loop region. The resulting data were combined with previous mtDNA data, a total of 95 mtDNA sequences were analyzed in order to estimate the relationships between Jomon and Yayoi people, and compared these sequences with modern east Asian populations. The analysis of DNA extracted from ancient skeletal materials has demonstrated that the Jomon people were closely related to mainland Japanese, Ryukyuans, Ainu, the people of the Korean Peninsula, Mongolian, and Buriat but almost no connection to Pacific people. Additionally, it has been shown that the Yayoi people did not have a close relationship to the Ainu or Ryukyu people, but instead held a close relationship to the people of the Korean Peninsula, Southern China, and mainland Japan, among others. According to a hypothesis based on analysis of modern mtDNA, a large portion of the genetic character of the mainland Japanese is the result of people coming overseas from the Asian continent after the Yayoi period. The result of this study is not consistent with the hypothesis extracted from modern sample.

现在，人线粒体基因组（mtDNA）的变异通过D-Loop区域的部分测序来推断人们的历史。我们从Jomon和Yayoi骨骼残留物中提取了DNA，并通过PCR扩增确定了其mtDNA D-Loop区域的核苷酸序列。将所得的数据与以前的mtDNA数据结合使用，总共分析了95个mtDNA序列，以估计乔蒙和Yayoi人之间的关系，并将这些序列与现代的东亚人群进行了比较。从古代骨骼材料中提取的DNA的分析表明，乔蒙人民与日本大陆，ryukyuans，ainu，朝鲜半岛，蒙古人和葬礼密切相关，但与太平洋人民几乎没有任何联系。此外，已经表明，Yayoi人与Ainu或Ryukyu人没有密切的关系，而是与朝鲜半岛，中国南部和日本大陆等人的人民建立了密切的关系。根据基于对现代mtDNA的分析的假设，日本大陆的遗传特征的很大一部分是人们在Yayoi时期从亚洲大陆出国来到海外的结果。这项研究的结果与从现代样本中提取的假设不一致。