Analysis of phylogenetic relationships among Triticeae grasses II - The (allo)polyploid genera

小麦科禾本科植物II-（异源）多倍体属的系统发育关系分析

• 批准号: 179096616
• 负责人: Dr. Frank R. Blattner
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/179096616?language=en
• 关键词: Analysis; phylogenetic; relationships; among; Triticeae

The grass tribe Hordeeae (till recently called Triticeae) includes the important cereals barley, rye and wheat, together with fodder grasses and ecologically important taxa of open landscapes. Thus, the taxa provide staple food for large parts of the human population. Although hypotheses about relationships of the different genera and species were erected for more than a century the phylogeny of the group is still not finally resolved. During the first project period we developed a DNA capture design that allows us to obtain and next-generation sequence >320 nuclear genes, uniformly distributed on the seven chromosomes of the members of the tribe. We used these loci to clarify species relationships of diploid Hordeeae taxa. Now we will analyze the polyploid taxa, to clarify mode of evolution and involvement of the different parental species of the allopolyploid genera of Hordeeae. A final phylogeny of the tribe will be obtained by sequence capture of the target loci, sequencing of pooled libraries on the Illumina platform, phasing of homoeologs, and coalescent-based phylogenetic analyses of the polyploid-derived sequences together with such already obtained from the diploid taxa. Our data will not only provide a phylogeny of all genera of Hordeeae but allow also insights in evolutionary dynamics of genome parts as well as estimation of the amount of introgression that is going on among Hordeeae taxa.

草族大麦草科（最近称为小麦科）包括重要的谷物大麦、黑麦和小麦，以及饲料草和开放景观中具有重要生态意义的类群。因此，该类群为大部分人口提供主食。尽管关于不同属和种之间关系的假设已经建立了一个多世纪，但该类群的系统发育仍未最终得到解决。在第一个项目期间，我们开发了一种 DNA 捕获设计，使我们能够获得下一代序列 > 320 个核基因，均匀分布在部落成员的 7 条染色体上。我们使用这些基因座来阐明二倍体大麦科类群的物种关系。 现在我们将分析多倍体类群，以阐明大麦科异源多倍体属的不同亲本物种的进化模式和参与。该部落的最终系统发育将通过目标位点的序列捕获、Illumina平台上的混合文库测序、同源同源物的定相以及对多倍体衍生序列以及已从二倍体类群获得的序列进行基于合并的系统发育分析来获得。我们的数据不仅将提供大麦科所有属的系统发育，而且还可以深入了解基因组部分的进化动力学，以及估计大麦科类群之间正在进行的基因渗入量。