DISSERTATION RESEARCH: Evolutionary Genetics of Novel Herbicide Tolerance in the Common Morning Glory

消灭有害生物的化学手段的引入加强了农业生产，但代价是抗性生物的迅速进化。我们对除草剂农达新耐受性进化的观察。在常见的牵牛花中，Ipomoea purpurea为人类介导的进化提供了一个极好的例子。我们从初步的温室研究中得到证据，表明紫穗槐母系对农达的耐受性存在遗传变异。我们将通过研究除草剂耐受性的遗传控制及其进化的潜在制约因素来进一步研究这一性状的进化。这些实验将通过温室杂交和随后的后代分析来检验耐受性是如何遗传的。在没有除草剂的情况下，将通过比较除草剂耐受性和易感个体的相对适合度来研究对耐受性进化的潜在限制。这项研究对美国农业的广泛影响是显而易见的。由于农达的使用正在显著增加，完成对其使用的进化分析至关重要。了解对这种除草剂的耐受性的遗传基础和适应度代价将具有广泛的重要性。

The introduction of chemical means of eradicating pest species intensified agricultural production, but at the cost of rapid evolution of resistant organisms. Our observation of the evolution of novel tolerance to the herbicide RoundUp. In the common morning glory, Ipomoea purpurea, provides an excellent example of human-mediated evolution. We have evidence from a preliminary greenhouse study that there is genetic variation among maternal lines of Ipomoea purpurea for tolerance to RoundUp. We will further investigate the evolution of this trait by studying the genetic control of herbicide tolerance and the potential constraints on its evolution. The experiments will examine how tolerance is inherited by performing greenhouse crosses and subsequent progeny analyses. The potential for constraints on the evolution of tolerance will be investigated by comparing relative fitness of herbicide tolerant and susceptible individuals in the absence of herbicide in the field. The broader impacts of this study are manifest to U.S. agriculture. Since use of RoundUp is increasing markedly, it is critical that evolutionary analyses of its use be completed. An understanding of the genetic basis and fitness costs of tolerance to this herbicide will be of broad importance.