Portable DNA-Probe-Array Synthesis System Using Acoustic-Wave Ejector and Atomizer Array

使用声波喷射器和雾化器阵列的便携式 DNA 探针阵列合成系统

• 批准号: 0310622
• 负责人: Eun Kim
• 金额: $ 24万
• 依托单位: University of Southern California
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0310622&HistoricalAwards=false
• 关键词: Portable; DNA; Probe; Array; Synthesis

For efficient and convenient DNA sequencing, we propose to develop a portable system for on-demand DNA probe-array synthesis, using two-dimensional MEMS ejector array (to shoot out DNA bases onto a DNA probe chip in any desired sequence) and atomizer array (to scatter away unbound DNA bases from the probe chip). The technical innovations of the proposed system are as follows: (1) the ejector array (unlike a nozzle-based ejector) can eject liquid droplets at any oblique angle, and does not have to be moved to ink a spot with four DNA bases, and (2) the atomizer array atomizes the unbound DNA bases (after a spotting step), and blows off the unwanted bases before moving to another spotting step for DNA synthesis, thus removing the costly step of the washing and drying steps. Also, we will integrate the 2-D ejector array with microchannels, chambers and other microfluidic handling components on a single silicon chip for a small, portable, affordable system. The portable DNA probe synthesis system will allow geneticists to produce any two dimensional array of any DNA sequence on a chip at their sites.One major research objective is to develop a flexible, low cost technology for an efficient genotyping to characterize hundreds of DNA samples for specific mutations for molecular genetic or molecular epidemiologic research. Genotyping is currently very time-consuming (and, therefore, very expensive), and it is timely to develop an efficient and flexible genotyping technique that allows formation of any DNA sequences on a glass chip on demand. The proposed activity will also include (1) research involvement of students from underrepresented groups (through active search of such applicants and more than usual mentoring of the selected students), (2) data and information dissemination through an internet web site that describes detailed information on DNA and protein microprobe-array synthesis on a chip surface through ink-jet printing of DNA bases or peptides, and (3) close collaboration with a research group (at USC's medical school) that specializes on DNA sequencing for genotyping and phenotyping for public health concerns.

为了有效和方便的DNA测序，我们建议开发一种便携式系统，用于按需DNA探针阵列合成，使用二维MEMS喷射器阵列（以任何所需的序列将DNA碱基射到DNA探针芯片上）和雾化器阵列（从探针芯片上散射掉未结合的DNA碱基）。该系统的技术创新点如下：（1）喷射器阵列（与基于雾化器的喷射器不同）可以以任何倾斜角度喷射液滴，并且不必移动以用四个DNA碱基墨点，以及（2）雾化器阵列雾化未结合的DNA碱基（在点样步骤之后），并且在移动到用于DNA合成的另一个点样步骤之前吹掉不需要的碱基，从而去除洗涤和干燥步骤的昂贵步骤。此外，我们还将把二维喷射器阵列与微通道、腔室和其他微流体处理组件集成在单个硅芯片上，形成一个小型、便携、负担得起的系统。便携式DNA探针合成系统将允许遗传学家在他们的现场在芯片上产生任何DNA序列的任何二维阵列。一个主要的研究目标是开发一种灵活的、低成本的技术，用于有效的基因分型，以表征数百个DNA样品的特定突变，用于分子遗传学或分子流行病学研究。基因分型目前非常耗时（因此非常昂贵），开发一种有效且灵活的基因分型技术，允许根据需要在玻璃芯片上形成任何DNA序列，这是及时的。拟议的活动还将包括（1）来自代表性不足群体的学生参与研究（通过积极寻找这样的申请人和比通常更多地指导选定的学生），（2）通过因特网网站传播数据和信息，该网站描述关于通过喷墨打印DNA碱基或肽在芯片表面上合成DNA和蛋白质微探针阵列的详细信息，（3）与南加州大学医学院的一个研究小组密切合作，该研究小组专门研究用于公共卫生问题的基因分型和表型的DNA测序。