Arabidopsis 2010: ATP: A Reverse Genetics Service for the Arabidopsis Community

需要多种工具来理解模式植物拟南芥的26，000个基因的功能。发现感兴趣的DNA序列中的诱导或自然变异极大地促进了对每个基因的分析。拟南芥TILLING项目（ATP）发现，测序和提供拟南芥研究人员订购的靶基因中的突变和多态性（自然变异）。对于每个靶基因座，ATP使用TILLING（靶向诱导的基因组局部病变）提供突变，TILLING是一种反向遗传学方法，可在化学诱变个体的群体中发现诱导的点突变。通过化学诱变可能发生的广泛的突变可以以不同的严重程度损害基因，从而提供有助于理解功能的等位基因系列。此外，对于每个目标基因座，ATP查询50个标准登记的天然多态性（Ecotilling）。因此，天然变体将可用于表型表征。第一个目标是TILL和ecotill每年180个基因。公众可在ATP网站（http：//tilling.fhcrc.org：9366/）输入订单。第二个目标是为本科生提供一个基于基因组研究的教学工具。一个教育链接将张贴在ATP网站，并将提供指导的机会，探索基因功能。可检索的包括TILLING系、突变和多态性，所有这些都可在TAIR基因组数据库网站（www.arabidopsis.org，参见“stocks”、“sequence viewer”和“allele/polymorphism”）上检索和排序。ATP提供的服务将通过提供一项重要的额外技术来补充现有的反向遗传学工具，从而促进拟南芥2010年的目标，以了解每个基因的功能。更广泛的影响除了丰富PI的教学和为华盛顿大学的本科生提供培训机会外，该项目还有一个具体的计划，让本科生进入四年制院校。与四年制大学的教师合作，我们正在开发本科教学练习，其中候选基因与选定表型的关系将由学生使用ATP的基因分型和靶向突变资源进行评估。过去十年在拟南芥中开发的基因组资源将被整合到这些练习中。因此，ATP将提供一个渠道，通过它，在小型四年制院校的本科生可以参与后基因组研究。

Multiple tools will be required to understand the function of the 26,000 genes of the model plant Arabidopsis. Discovering induced or natural variation in DNA sequences of interest greatly facilitates the analysis of each gene. The Arabidopsis TILLING Project (ATP) discovers, sequences and delivers mutations and polymorphisms (natural variations) in target genes ordered by Arabidopsis researchers. For each target locus ATP provides mutations using TILLING (Targeting Induced Local Lesions In Genomes), a reverse genetics method that discovers induced point mutations in populations of chemically mutagenized individuals. The broad range of mutations possible by chemical mutagenesis can impair a gene with different severity, thus providing an allelic series useful in understanding function. In addition, for each target locus ATP queries 50 standard accessions for natural polymorphisms (Ecotilling). Natural variants will thus be available for phenotypic characterization. The first objective is to TILL and ecotill 180 genes per year. Orders can be entered by the public at the ATP site (http://tilling.fhcrc.org:9366/). The second objective is to provide to undergraduate students an Arabidopsis-based educational tool on genomic research. An education link will be posted at the ATP site and will provide guided opportunities to explore gene function. Deliverables include TILLING lines, mutations and polymorphisms, all available for search and ordering at the TAIR Arabidpsis database site (www.arabidopsis.org, see "stocks", "sequence viewer" and "allele/polymorphisms"). The service provided by ATP will supplement the existing reverse genetics tools by providing an important additional technology, thus facilitating the Arabidopsis 2010 objective to understand the function of each gene. Broader impactIn addition to enriching the teaching of the PIs and providing training opportunity for undergraduates at the University of Washington, this project has a specific plan to involve undergradues in 4-year institutions. In collaboration with instructors from four-year universities we are developing exercises for undergraduate teaching in which the relationship of candidate genes to selected phenotypes will be assessed by students using the genotyping and targeted mutations resources of ATP. The genomic resources developed over the past decade in Arabidopsis will be integrated into these exercises. Therefore, ATP will provide a conduit through which undergraduate students in small four-year institutions can participate in postgenomic research.