(B7N) - A Low Impact, High Efficiency Transformation Marker System

选择是遗传分析和植物转化的重要工具。 最广泛使用的选择方法涉及对抗生素如卡那霉素或除草剂如BASTA的抗性。 虽然这些选择工具相当有效，但存在毒性以及监管和公众接受度低的问题。 EAGER项目旨在开发一种新的基于顶端分生组织细胞特性改变的作物选择标记系统。该系统将基于通过转基因补充选定的发育突变体中的非必需缺陷。该系统将提供多种优势，包括较低的细胞毒性，更容易应用靶向修饰，降低标记物逃逸的风险，以及提高消费者接受度和法规遵从性。 目的1将使用一个特征的拟南芥调控途径，以证明基本的可行性。 目标2将在拟南芥、番茄和水稻中鉴定和补充新基因的突变，以测试它们对该系统的适用性。 最后的目标3将探索使用正向筛选在表现出特定表型的植物中鉴定合适的突变。该项目希望在拟南芥模式系统中展示这种新系统的应用，然后将其应用扩展到至少一种作物物种。 自从近30年前植物转化的最初报道以来，对能够克服抗生素/除草剂抗性缺点的选择标记的搜索一直很激烈。这种标记系统有可能彻底改变植物科学家在基础研究和作物改良中进行转化的方式。本文提出的研究采用了一种全新的方法，将使用天然存在的植物基因;因此，通过这种方法生产的产品将更有可能被消费者接受并获得监管机构的批准。 如果成功，这些方法将在实验室网站http://comailab.genomecenter.ucdavis.edu/index.php/上公布。 植物材料将存放在TAIR或番茄遗传资源合作社和其他适当的公共种质库。

Selection is an important tool for genetic analysis and plant transformation. The most widely used selection approach involves resistance to antibiotics such as kanamycin or to herbicides such as BASTA. Although quite effective, these selection tools present problems involving toxicity as well as low regulatory and public acceptability. This EAGER project proposes to develop a new selectable marker system for crop plants based on the alteration of cellular properties within the apical meristems. The system will be based on complementing through transgenes non-essential deficiencies in selected developmental mutants. The system will provide multiple advantages including lower cytotoxicity, easier application of targeted modifications, reduced risk of marker escape, and improved consumer acceptance and regulatory compliance. Aim 1 will use a characterized Arabidopsis regulatory pathway to demonstrate basic feasibility. Aim 2 will identify and complement mutations in new genes in Arabidopsis, tomato and rice to test their suitability for this system. The final aim 3 will explore the use of forward screens for identifying suitable mutations among plant exhibiting specific phenotypes. The project expects to demonstrate the application of this novel system in the Arabidopsis model system and then extend its use to at least one crop species. Since the original reports of plant transformation nearly 30 years ago, the search for selectable markers that would overcome the drawbacks of antibiotic/herbicide resistance has been intense. This marker system has the potential to revolutionize the way plant scientists approach transformation for basic research and for crop improvement. The research proposed here follows a radically new approach and will use naturally existing plant genes; thus, products generated by this method will more likely be accepted by consumers and approved by regulatory agencies. If successful, the methods will be made available on the laboratory's web site at http://comailab.genomecenter.ucdavis.edu/index.php/ Data methods. Plant materials will be deposited in TAIR or Tomato Genetic Resource Cooperative and other appropriate public germplasm repository.