Synthesis, Characterization and Application of Luminescent Nanoparticles for Single Cell Analysis

单细胞分析用发光纳米粒子的合成、表征及应用

• 批准号: 0314027
• 负责人: Matthew Tarr
• 金额: $ 38.5万
• 依托单位: University of New Orleans
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-15 至 2008-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0314027&HistoricalAwards=false
• 关键词: Synthesis; Characterization; Application; Luminescent; Nanoparticles

Professor Zeev Rosenzweig of the University of New Orleans is funded by the Analytical and Surface Chemistry Program to develop a new generation of fluorescence nanosensors suitable for intracellular measurements with high sensitivity, selectivity and reproducibility. One approach is to miniaturize lipobeads, which are polymer nanoparticles coated with a layer of phospholipids, to produce sensors for glutathione, chloride and nitric oxide. In a second objective, fluorescence resonance energy transfer (FRET) based interactions between fluorescent nanoparticles and drug molecules will be used to monitor drug delivery into neurons. The in situ, temporal and spatial mapping of intracellular concentrations of specific target molecules is of enormous priority in biology. Present reporter molecules are sometimes inadequate to detect certain key molecules and drugs non-invasively. The ability to monitor the locale and timing of analytes in living cells can contribute to the understanding of how they work and the effects of physiological conditions on them.

新奥尔良大学的Zeev Rosenzweig教授由分析和表面化学计划资助，开发新一代荧光纳米传感器，适用于具有高灵敏度，选择性和重现性的细胞内测量。 一种方法是将脂质珠（lipobeads）（一种涂有一层磷脂的聚合物纳米颗粒）包裹起来，以产生谷胱甘肽、氯化物和一氧化氮的传感器。 在第二个目标中，基于荧光纳米颗粒和药物分子之间的相互作用的荧光共振能量转移（FRET）将用于监测药物递送到神经元中。 特定靶分子的细胞内浓度的原位、时间和空间映射在生物学中具有巨大的优先权。 目前的报告分子有时不足以非侵入性地检测某些关键分子和药物。 监测活细胞中分析物的位置和时间的能力有助于了解它们如何工作以及生理条件对它们的影响。