Quantum Dots FRET-Based Probes for Cellular Assays

用于细胞检测的基于 FRET 的量子点探针

• 批准号: 0717526
• 负责人: Matthew Tarr
• 金额: $ 21万
• 依托单位: University of New Orleans
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0717526&HistoricalAwards=false
• 关键词: Quantum; Dots; FRET; Based; Probes

In this two-year award, funded by the Experimental Physical Chemistry Program of the Division of Chemistry, Professor Matthew A. Tarr and Prof. Zeev Rosenzweig of the University of New Orleans, along with their graduate and undergraduate research students, plan to synthesize protected CdSe/ZnS quantum dots to be used for intracellular chemical assays. The PIs and their team will study protease activity in living cells, and will develop microfluidic methods for their whole-cell assays, in collaboration with colleagues at NIST-Gaithersburg . The ultimate goal of these studies is to develop quantitative methods for intracellular chemical imaging.Besides the broader scientific and technological impact of this research, Profs. Tarr and Rosenzweig will provide their diverse group of research students with a unique interdisciplinary graduate research experience. The PIs will also develop outreach activities with their graduate students to provide one-to-one mentoring between UNO graduate students and high school students in New Orleans.

这项为期两年的奖项由化学系实验物理化学项目资助，新奥尔良大学的Matthew A.Tarr教授和Zeev Rosenzweig教授与他们的研究生和本科生计划合成受保护的CdSe/ZnS量子点，用于细胞内化学分析。PIs和他们的团队将研究活细胞中的蛋白酶活性，并将与NIST-Gaithersburg的同事合作，开发用于全细胞分析的微流控方法。这些研究的最终目标是开发细胞内化学成像的定量方法。除了这项研究的更广泛的科学和技术影响，教授。塔尔和罗森茨韦格将为他们不同的研究型学生群体提供独特的跨学科研究生研究体验。专业人员还将与其研究生开展外联活动，在联合国组织研究生和新奥尔良的高中生之间提供一对一的指导。