PEET: Bacterial Taxonomy: Classical and Molecular-Based Monographic Research on the Aquificales

PEET：细菌分类学：关于 Aquificales 的经典和分子专题研究

• 批准号: 0328326
• 负责人: Anna-Louise Reysenbach
• 金额: $ 75万
• 依托单位: Portland State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-11-01 至 2009-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0328326&HistoricalAwards=false
• 关键词: PEET; Bacterial; Taxonomy; Classical; Molecular

Research and training in bacterial taxonomy will be supported through the Partnerships for Enhancing Expertise in Taxonomy activity, under the supervision of Prof. Reysenbach at Portland State University and her colleagues in the U.S. and abroad. Training in classical and molecular genome-based approaches will focus on bacteria of the order Aquificales, known from hydrothermal environments around the world, including marine vents and terrestrial hotsprings. These bacteria support their growth through novel oxidative and reductive reactions utilizing minerals, usually in very hot environments, and are hence known as chemolithoautotrophs. The laboratory culturing of these organisms, for detailed metabolic studies, is difficult and time consuming, and Prof. Reysenbach is one of the few experts in the world knowledgeable and successful in this enterprise. Cultures and collections from all over the world have been assembled for her use and that of her students, and will be augmented with additional materials from colleagues exploring marine and hotspring localities in Yellowstone and in Russia, New Zealand, the Azores, and elsewhere. Whole-genome sequencing of bacteria is producing a wealth of DNA sequence and gene data, including members of the Aquificales, and the comparative analysis of these data enables reconstruction of phylogenetic relationships among the genes and the organisms and determination of the peculiar metabolic activities that permit them to live and diversify in extreme environments. Students will learn a full range of technologies for comparative phylogenomics and for modern bacterial systematics, as well as novel culturing procedures. As well, short courses targeted to school teachers and the public under the "Yellowstone Microbes" program will contribute to ongoing education in modern microbial taxonomy.

在波特兰州立大学的Reysenbach教授及其在美国和国外的同事的监督下，细菌分类学的研究和培训将通过加强分类学活动专业知识的伙伴关系得到支持。传统方法和基于分子基因组的方法方面的培训将侧重于水生目细菌，这种细菌来自世界各地的热液环境，包括海洋喷口和陆地温泉。这些细菌通常在非常热的环境中通过利用矿物质的新型氧化和还原反应来支持它们的生长，因此被称为化能无机自养生物。这些生物体的实验室培养，用于详细的代谢研究，是困难和耗时的，Reysenbach教授是世界上少数几个在这方面知识渊博和成功的专家之一。来自世界各地的文化和收藏品已被收集起来供她和她的学生使用，并将增加来自黄石公园和俄罗斯，新西兰，亚速尔群岛和其他地方的海洋和温泉地区的同事的额外材料。 细菌的全基因组测序正在产生丰富的DNA序列和基因数据，包括水生生物目的成员，这些数据的比较分析能够重建基因和生物体之间的系统发育关系，并确定使它们能够在极端环境中生活和多样化的特殊代谢活动。学生将学习比较细菌基因组学和现代细菌系统学的全方位技术，以及新的培养程序。此外，在“黄石微生物”计划下针对学校教师和公众的短期课程将有助于现代微生物分类学的持续教育。