SGER: Development of a Perfused Heart Model for Heat Shock Protein Preconditioning

SGER：开发用于热休克蛋白预处理的灌注心脏模型

• 批准号: 0332052
• 负责人: Kenneth Diller
• 金额: $ 6.51万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2005-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0332052&HistoricalAwards=false
• 关键词: SGER; Development; Perfused; Heart; Model

Extensive empirical data within the medical and scientific communities indicate that cells subjected to mild thermal stress will over-express heat shock proteins (HSP), which are known to have protective and reparative effects on injured cells and tissues. We have completed extensive quantitative measurements of the thermal stimulation conditions optimal to HSP expression in cell cultures. This proposal presents a unique set of experiments designed to take this technology to the next level of development by establishing a new protocol for producing and measuring HSP expression in a whole organ model. The experiments are based on an existing perfused working rat heart model developed and used by one of our medical school colleagues for more than two decades. Thermal stress will be produced by irradiation with a microwave source, which is well suited for adaptation to the perfusion rig and to producing a uniform energy absorption field throughout a rat heart. The focus of this first aspect of the whole organ study is to determine the effectiveness of measuring HSP production in a rat heart and of extending the duration of the perfusion trial to as long a period as possible. We will implement novel measurement techniques to quantify the temporal thermal field, via multiplanar magnetic resonance temperature imaging (MRTI), in the heart. Although we have several decades of experience in bioheat transfer research in our laboratory, this project is the initial foray into a brand new domain of study in which there is no existing experience anywhere in the world (to our knowledge.) Recent developments in the field of molecular biology have made this work possible, and the project has a huge potential eventual payoff in medical applications. The award has been funded by the Thermal Transport and Thermal Processing Program of the Chemical and Transport Systems Division.

医学和科学界的大量经验数据表明，受到轻度热应激的细胞将过度表达热休克蛋白（HSP），已知其对受损细胞和组织具有保护和修复作用。 我们已经完成了广泛的定量测量的热刺激条件下的最佳HSP表达的细胞培养。 该提案提出了一套独特的实验，旨在通过建立一种新的方案来生产和测量整个器官模型中HSP的表达，将这项技术推向下一个发展水平。 这些实验是基于现有的灌注工作大鼠心脏模型，该模型由我们的一位医学院同事开发和使用了二十多年。 热应力将通过用微波源照射来产生，微波源非常适合于适应灌注装置并在整个大鼠心脏中产生均匀的能量吸收场。 整个器官研究的第一个方面的重点是确定测量大鼠心脏中HSP产生的有效性，以及将灌注试验的持续时间延长到尽可能长的时间。 我们将实施新的测量技术，以量化的时间热场，通过多平面磁共振温度成像（MRTI），在心脏。 虽然我们在实验室的生物传热研究方面有几十年的经验，但这个项目是对一个全新的研究领域的首次尝试，在这个领域中，世界上任何地方都没有现有的经验（据我们所知）。 分子生物学领域的最新发展使这项工作成为可能，该项目在医学应用中具有巨大的潜在最终回报。该奖项由化学和运输系统部门的热运输和热处理计划资助。