Microbial Genome Sequencing: Complete Genomic Sequencing of Arthrobacter aurescens strain TC1

微生物基因组测序：金节杆菌 TC1 菌株的完整基因组测序

• 批准号: 0333161
• 负责人: Michael Sadowsky
• 金额: $ 69.92万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-15 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0333161&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Complete; Genomic

A grant has been awarded to Drs. Michael Sadowsky and Lawrence Wacket at the University of Minnesota, Dr. Patrick Hamilton at the Science Museum of Minnesota, and Dr. Karen Nelson at The Institute for Genome Research (Maryland) to obtain the complete DNA sequence of and identify the major genes in the soil bacterium Arthrobacter aurescens strain TC1. Arthrobacter strains are widespread in soil around the globe and contribute to recycling organic matter and breaking down environmental pollutants. Despite their environmental importance, no member of this group has had its total DNA content sequenced. Modern DNA sequencing technologies and computer tools will be used to obtain sequence information and the potential function of all the genes, including those involved in ridding the environment of harmful chemicals. Drs. Sadowsky and Wackett will partner with the Science Museum of Minnesota (SMM) in St. Paul, MN, which hosts more than one million visitors annually, to develop exhibits that display the genomic information obtained in this project and its importance for the environment.The availability of complete microbial genome sequences is revolutionizing microbiology, medicine, and environmental sciences. Genome sequencing provides information on how bacterial genes are organized on their chromosome, what genes are present, and how they evolved. Arthrobacter are unique bacteria in that they have relatively complex life cycles, survive in extreme environments, such as the arctic, and are extremely resistant to drying and starvation. These bacteria are capable of degrading a variety of toxic substances in the environment, including pesticides, gasoline additives, explosives, and industrial solvents. The genomic sequence of Arthrobacter aurescens will provide invaluable information concerning this microorganism's fundamentally important biological properties. Given their ability to break down a large number of environmentally relevant compounds, knowledge obtained from the complete genomic sequence will help guide the use of these microbes in the bioremediation of contaminated environments.

明尼苏达大学的Michael Sadowsky博士和Lawrence Wacket博士、明尼苏达科学博物馆的帕特里克汉密尔顿博士和基因组研究所（马里兰州）的Karen纳尔逊博士获得了一笔赠款，以获得土壤细菌节杆菌菌株TC 1的完整DNA序列并确定其主要基因。 节杆菌属菌株广泛存在于地球仪的土壤中，有助于有机物质的再循环和环境污染物的分解。尽管它们对环境很重要，但这一组中没有一个成员的总DNA含量被测序。现代DNA测序技术和计算机工具将用于获得所有基因的序列信息和潜在功能，包括那些参与消除环境中有害化学物质的基因。Sadowsky博士和Wackett博士将与明尼苏达州圣保罗的明尼苏达科学博物馆（SMM）合作，开发展示该项目获得的基因组信息及其对环境的重要性的展览。完整的微生物基因组序列的可用性正在彻底改变微生物学，医学和环境科学。基因组测序提供了关于细菌基因如何在染色体上组织，存在哪些基因以及它们如何进化的信息。节杆菌是一种独特的细菌，它们具有相对复杂的生命周期，能在极端环境中生存，如北极，并且对干燥和饥饿具有极强的抵抗力。这些细菌能够降解环境中的各种有毒物质，包括杀虫剂，汽油添加剂，爆炸物和工业溶剂。金黄节杆菌的基因组序列将提供有关这种微生物的基本重要的生物学特性的宝贵信息。鉴于它们能够分解大量与环境相关的化合物，从完整的基因组序列中获得的知识将有助于指导这些微生物在污染环境的生物修复中的使用。