Microbial Genome Sequencing: Genome Sequencing of Agrobacterium biovar Type Strains

微生物基因组测序：农杆菌生物变型菌株的基因组测序

• 批准号: 0333297
• 负责人: Eugene Nester
• 金额: --
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-10-01 至 2007-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0333297&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Agrobacterium; biovar

The goal of this project is to produce annotated genome sequences for two biovars of Agrobacterium; the biovar II strain, A. radiobacter K84 and the biovar III strain, A. vitis S4. Previous analysis of the biovar I representative of this diverse species provided intriguing insights into the evolutionary relationship shared between this pathogen and symbionts of the Rhizobiaceae. Our choice of K84 as the bv II representative is based on the large amount of published data available regarding the field properties of this strain as well as its position as the single most effective biological control agent available for any bacterial disease of plants. The bv III representative, S4, was chosen based on a number of intriguing biological properties including its narrow host range, its unique T-DNA composition and organization as well as its ability to produce a hypersensitive response on non-host plants via a mechanism that is apparently distinct from that of other phytopathogens. This project brings together expertise from five independent groups with significant experience in Agrobacterium biology, genomics and undergraduate education. A key portion of this project is geared towards providing a training platform in genomics technology for undergraduate researchers at different participating institutions. Such participation will both facilitate our project goals and provide key training for new scientists. Access to the genome sequence of these organisms will allow extensive comparative genomic analyses, relate genotype to field biology and behavior, and facilitate the molecular characterization of biological control, host range and the unique factors mediating the hypersensitive response.

该项目的目标是为农杆菌的两个生物变体产生带注释的基因组序列；生物多样性II菌株为放射杆菌K84，生物多样性III菌株为葡萄球菌S4。以前对这种多样化物种的生物变种I代表的分析为这种病原体和根瘤菌科共生体之间的进化关系提供了有趣的见解。我们选择K84作为bv II的代表是基于大量关于该菌株田间特性的公开数据，以及它作为任何植物细菌性疾病最有效的单一生物防治剂的地位。bv III的代表，S4，被选中是基于许多有趣的生物学特性，包括其狭窄的寄主范围，其独特的T-DNA组成和组织，以及它通过一种明显不同于其他植物病原体的机制对非寄主植物产生超敏反应的能力。该项目汇集了在农杆菌生物学、基因组学和本科教育方面具有丰富经验的五个独立小组的专业知识。该项目的一个关键部分是为不同参与机构的本科生研究人员提供基因组学技术培训平台。这样的参与既有利于我们的项目目标，也为新科学家提供了关键的培训。获得这些生物的基因组序列将允许广泛的比较基因组分析，将基因型与野外生物学和行为联系起来，并促进生物控制，宿主范围和介导超敏反应的独特因素的分子表征。