Microbial Genome Sequencing: Aeromonas Hydrophila Under the Auspices of the Microbial Genome Sequencing Program

微生物基因组测序：微生物基因组测序计划支持下的嗜水气单胞菌

• 批准号: 0334247
• 负责人: Amy Martin-Carnahan
• 金额: --
• 依托单位: University of Maryland at Baltimore
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-10-01 至 2007-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0334247&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Aeromonas; Hydrophila

A grant has been awarded to Drs. Amy Martin-Carnahan, Sam Joseph and Colin Stine at the University of Maryland, Jonathan Heidelberg at The Institute for Genomic Research and Ashok K. Chopra at the University of Texas to produce the complete genome sequence of the bacterium Aeromonas hydrophila. This will provide a roadmap for understanding the physiology and ecology of this microorganism which is found in all manner of aquatic environments worldwide, is an important fish pathogen and interacts with many other microorganisms in the aquatic environment. The DNA of the organism will be isolated, purified and extracted and each fragment will be sequenced and subsequently the complete genetic code will be pieced together by a combination of state-of-the art molecular and computational techniques. This study will provide vital genetic information about this microbe, and its role in the environment and interactions with other organisms. Aeromonas hydrophila is on the Drinking Water Contaminant Candidate List of the U.S. Environmental Protection Agency, and is listed as a pathogen of concern in the U.S. Food & Drug Administration's "Bad Bug Book". Overall, the sequencing of A. hydrophila will further our understanding of this and related organisms in many of the major aquatic ecosystems on this planet. With our increasing dependence on the aquatic environment, it is important for us to better understand the overall composition of its microbiota, thus allowing us to detect changes in environmental quality that may pose hazards to human and ecosystem health. The results of this project will be made available to the general scientific community as well as incorporated into undergraduate and graduate science courses at various campuses of the University of Maryland. The sequence data will also be available through scientific databases and web pages as well as other public databases.

马里兰州大学的艾米·马丁·卡纳汉博士、萨姆·约瑟夫博士和科林·斯廷博士、基因组研究所的乔纳森·海德堡博士和阿肖克·K.得克萨斯大学的Chopra，以产生细菌Aeromonasericata的完整基因组序列。这将为了解这种微生物的生理和生态提供路线图，这种微生物存在于世界各地的各种水生环境中，是一种重要的鱼类病原体，并与水生环境中的许多其他微生物相互作用。将对生物体的DNA进行分离、纯化和提取，并对每个片段进行测序，随后将通过最先进的分子和计算技术相结合，将完整的遗传密码拼凑起来。这项研究将提供有关这种微生物的重要遗传信息，以及它在环境中的作用和与其他生物的相互作用。嗜水气单胞菌在美国环境保护署的饮用水污染物候选名单上，并在美国食品药品监督管理局的“Bad Bug Book”中被列为关注的病原体。 总的来说，A.这将进一步加深我们对这个星球上许多主要水生生态系统中的这种生物和相关生物的理解。随着我们对水生环境的依赖越来越大，我们必须更好地了解其微生物群的整体组成，从而使我们能够检测可能对人类和生态系统健康造成危害的环境质量变化。 该项目的结果将提供给一般科学界，并纳入马里兰州大学各校区的本科生和研究生科学课程。序列数据也将通过科学数据库和网页以及其他公共数据库提供。