Reconstruction and Annotation of Transcribed Sequences in Plants

0338709 Quackenbush基因组研究所（TIGR）已获得一笔赠款，用于扩大和继续开发TIGR植物基因索引（http：//www.tigr.org/tdb/tgi.shtml）。 表达序列标签（EST）的测序提供了许多生物体中转录序列集合的第一个一瞥，并且通过对EST数据的彻底分析可以获得重要的额外信息。 目前，总指数代表了60多个物种：19种植物，22种动物，13种原生生物和7种真菌。该项目的继续将使TIGR能够继续向社区提供这些数据库和资源（免费），并继续开发其他功能，这些功能将增强目前提供的注释，包括识别SNP，预测微阵列的70-mer序列集合，以及创建Resourcer工具的植物版本。这些数据库是使用高度精炼和准确的计算协议产生的，该协议旨在重建由数据表示的转录本，为这些重建的序列分配初步注释，将它们映射到越来越多的完全测序的基因组中，并鉴定直向同源基因。结果通过一个易于访问的网络界面提供，并提出了额外的分析，包括鉴定推定的直向同源基因。因此，TGIR指数为研究界提供了宝贵的资源，并为NSF和其他联邦机构资助的许多正在进行的项目提供了对EST的分析。

0338709 Quackenbush The Institute for Genomic Research (TIGR) has been awarded a grant to expand and continue development of the TIGR Plant Gene Indices (http://www.tigr.org/tdb/tgi.shtml). Sequencing of Expressed Sequence Tags (ESTs) has provided a first glimpse of the collection of transcribed sequences in many organisms, and significant additional information can be obtained by a thorough analysis of the EST data. The total indices currently represent more than 60 species: 19 plant species, 22 animal species, 13 protists, and 7 fungal species. The continuation of this project will allow TIGR to continue to provide these databases and resources to the community (free of charge) and to continue development of additional features that will enhance the annotation currently provided, including the identification of SNPs, prediction of a collection of 70-mer sequences for microarrays, and the creation of a plant version of the Resourcer tool. These databases are produced using highly refined and accurate computational protocols designed to reconstruct transcripts represented by the data, to assign preliminary annotation to those reconstructed sequences, to map them to an increasing number of completely sequenced genomes, and to identify orthologous genes. The results are provided through an easily accessible web-interface and that presents additional analysis, including the identification of putative orthologous genes. As such, the TGIR indices provide a valuable resource for the research community and providing analysis of ESTs for many ongoing projects funded by the NSF and other federal agencies.