Reconstruction and Annotation of Transcribed Sequences in Plants

0338709奎肯布什基因组研究所获得了一笔赠款，用于扩大和继续开发基因组研究所的植物基因指数(http://www.tigr.org/tdb/tgi.shtml).表达序列标签(EST)的测序提供了对许多生物体转录序列收集的第一次窥探，通过对EST数据的彻底分析可以获得重要的额外信息。总指数目前代表了60多个物种：19个植物物种、22个动物物种、13个原生动物和7个真菌物种。该项目的继续进行将使TIGR能够继续(免费)向社区提供这些数据库和资源，并继续开发其他功能，以加强目前提供的注释，包括识别SNPs、预测用于微阵列的70聚体序列集合，以及创建植物版的资源分析工具。这些数据库是使用高度精细和准确的计算协议来产生的，这些协议旨在重建由数据表示的转录本，为这些重建的序列分配初步注释，将它们映射到越来越多的完全测序的基因组，并识别直源基因。结果是通过一个易于访问的网络界面提供的，并提供了额外的分析，包括对假定的同源基因的鉴定。因此，TGIR指数为研究界提供了宝贵的资源，并为NSF和其他联邦机构资助的许多正在进行的项目提供了EST分析。

0338709 Quackenbush The Institute for Genomic Research (TIGR) has been awarded a grant to expand and continue development of the TIGR Plant Gene Indices (http://www.tigr.org/tdb/tgi.shtml). Sequencing of Expressed Sequence Tags (ESTs) has provided a first glimpse of the collection of transcribed sequences in many organisms, and significant additional information can be obtained by a thorough analysis of the EST data. The total indices currently represent more than 60 species: 19 plant species, 22 animal species, 13 protists, and 7 fungal species. The continuation of this project will allow TIGR to continue to provide these databases and resources to the community (free of charge) and to continue development of additional features that will enhance the annotation currently provided, including the identification of SNPs, prediction of a collection of 70-mer sequences for microarrays, and the creation of a plant version of the Resourcer tool. These databases are produced using highly refined and accurate computational protocols designed to reconstruct transcripts represented by the data, to assign preliminary annotation to those reconstructed sequences, to map them to an increasing number of completely sequenced genomes, and to identify orthologous genes. The results are provided through an easily accessible web-interface and that presents additional analysis, including the identification of putative orthologous genes. As such, the TGIR indices provide a valuable resource for the research community and providing analysis of ESTs for many ongoing projects funded by the NSF and other federal agencies.