An ecophysiological analysis of marine facultatively lithotrophic bacteria

海洋兼性食石细菌的生态生理学分析

• 批准号: 0742053
• 负责人: Gary King
• 金额: --
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-30 至 2009-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0742053&HistoricalAwards=false
• 关键词: ecophysiological; analysis; marine; facultatively; lithotrophic

Drs. King and Tolli will analyze the significance, structure and function of bacterial facultative lithotroph and mixotroph (FL/M) communities along a near-shore to offshore transect in the water column of the marine Damariscotta River and the Gulf of Maine, and in intertidal sediments proximate to a near-shore sites. The investigators suggest that FL/M communities play important and poorly appreciated roles in heterotrophic carbon metabolism, as well as in the dynamics of carbon monoxide (CO), hydrogen and other reduced inorganic substrates. These researchers will investigate FL/M using molecular tools for the large sub-unit genes of form IA and IC bacterial ribulose-1,5-bisphosphate carboxylase/ oxygenase (rbcL), and CO dehydrogenase (coxL) that they have recently developed, and that they will apply for assays of marine systems for the first time. CoxL will provide insights regarding the distribution and diversity of some mixotrophs and facultative lithotrophs; rbcL will provide insights regarding both facultative and obligate lithotrophs. Recent analyses of various lithotrophic bacterial rbcL sequences and rbcL sequences from environmental DNA indicate that they can use phylogenetic tools to distinguish between facultative and obligate lithotrophs, thus allowing the PIs to answer questions about lithotrophic community structure that could not be addressed previously. In addition, these researchers will determine what relationships exist, if any, between the structure and diversity of FL/M communities and microbial abundance, cellular respiratory activity (ETS activity), and transformations of CO, hydrogen, ammonium and thiosulfate. The broader impacts of the proposed study will be derived from the investigator's efforts to train a postdoctoral research associate, Ph.D. student and undergraduate students in aspects of marine biogeochemistry, the physiology and ecology of specific groups of marine microbes and in molecular microbial ecology. In addition, they will contribute to a greater understanding of the Gulf of Maine and its near-shore ecosystems, both of which are ecologically and economically important a regional scales.

King和Tolli博士将分析细菌兼性石养生物和混合营养生物（FL/M）群落的意义、结构和功能，这些群落沿着一个从近岸到近海的断面，分布在海洋Damascotta河和缅因州湾的水柱中，以及靠近近岸地点的潮间带沉积物中。 研究人员认为，FL/M社区在异养碳代谢以及一氧化碳（CO），氢和其他还原无机基质的动态中发挥重要而不受重视的作用。 这些研究人员将使用他们最近开发的IA型和IC型细菌核酮糖-1，5-二磷酸羧化酶/加氧酶（rbcL）和CO脱氢酶（coxL）的大亚基基因的分子工具来研究FL/M，并且他们将首次应用于海洋系统的检测。 CoXL将提供有关一些混合营养体和兼性石养生物的分布和多样性的见解; rbcL将提供有关兼性和专性石养生物的见解。 最近的各种石养细菌rbcL序列和rbcL序列从环境DNA的分析表明，他们可以使用系统发育的工具来区分兼性和专性石养生物，从而使PI回答有关石养群落结构的问题，以前不能解决。 此外，这些研究人员将确定FL/M群落的结构和多样性与微生物丰度、细胞呼吸活性（ETS活性）以及CO、氢、铵和硫代硫酸盐的转化之间存在何种关系（如果有的话）。 拟议研究的更广泛影响将来自研究者培训博士后研究助理的努力。为学生和本科生提供海洋生物地球化学、特定海洋微生物群体的生理学和生态学以及分子微生物生态学方面的课程。 此外，他们将有助于更好地了解缅因州湾及其近岸生态系统，这两者都是生态和经济上重要的区域尺度。