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Regulation of Flower Maturation

Regulation of Flower Maturation
花成熟的调节
批准号:
0344257
负责人:
Jason Reed
金额:
$40.58万
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
2004
资助国家:
美国
项目状态:
已结题
起止时间:
2004-01-15 至 2007-05-31

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中文摘要
翻译
花成熟的调控本计画以模式植物拟南芥为研究对象,探讨花成熟的机制。 缺乏称为ARF 6和ARF 8的两种转录调节因子的突变植物具有在未成熟阶段阻止其生长的花,因此这些花保持为不育的闭合芽。 弱光条件也会导致类似阶段的停滞,这表明光可能通过ARF 6和ARF 8调节花的成熟。 为了探索花成熟是如何被调节的机制细节,里德博士将首先建立精确的光照条件,使我们能够控制花的成熟。 然后,他将研究这些光照条件如何影响ARF 6和ARF 8蛋白的丰度,以及编码它们的基因的表达。 最后,他将研究一种称为miR 167(micro-RNA 167)的天然小RNA分子的作用,该分子预计可调节ARF 6和ARF 8的翻译或转录稳定性。 他将在各种光照条件下测量miR 167的水平,检查它在哪些组织中对报告基因构建体有活性,并确定过量产生miR 167或使ARF 6对其活性免疫对花成熟的影响。 与ARF 6,ARF 8和miR 167密切相关的基因存在于单子叶植物中,因此这些研究中揭示的调控机制可能是所有开花植物所共有的。 了解这些机制可能允许操纵或改进具有可食用花蕾的作物,如西兰花或丁香。
英文摘要
Regulation of flower maturationThis project is to study mechanisms by which flowers mature in the model plant Arabidopsis. Mutant plants deficient in two transcriptional regulators called ARF6 and ARF8 have flowers that arrest their growth at an immature stage, and these flowers therefore remain as closed buds that are infertile. Low light conditions also cause arrest at a similar stage, suggesting that light might regulate flower maturation through ARF6 and ARF8. To explore in mechanistic detail how flower maturation is regulated, Dr. Reed will first establish precise light conditions that allow us to control flower maturation. He will then examine how these light conditions affect abundance of ARF6 and ARF8 proteins, and expression of the genes encoding them. Finally, he will examine the role of a naturally occurring small RNA molecule called miR167 (micro-RNA 167) that is predicted to regulate ARF6 and ARF8 translation or transcript stability. H e will measure the level of miR167 under various light conditions, examine in which tissues it is active against a reporter gene construct, and determine the effect on flower maturation of overproducing miR167 or of rendering ARF6 immune to its activity. Genes closely related to ARF6, ARF8, and miR167 are present in monocots, so the regulatory mechanisms revealed in these studies may be common to all flowering plants. Understanding these mechanisms may allow manipulation or improvement of crops with edible flower buds such as broccoli or cloves.
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Collaborative Research: Hormonal control of stamen filament growth
Collaborative Research: Bilateral BBSRC-NSF/BIO: Regulation of plant stomatal aperture by SAUR (Small Auxin Up RNA) proteins
Auxin Response Factors in flower maturation
Functions of Arabidopsis SAUR proteins
国内基金
海外基金
MYB转录因子SINGLE FLOWER调控番茄果实数目的分子机制
萱草花开放时间(Flower Opening Time)的生物钟调控机制研究
  • 批准号:
    31971706
  • 项目类别:
    面上项目
  • 资助金额:
    59.0万元
  • 批准年份:
    2019
  • 负责人:
    高亦珂
  • 依托单位:
AGAMOUS-LIKE FLOWER分子调控网络在豆科植物花发育中的功能研究
  • 批准号:
    31970329
  • 项目类别:
    面上项目
  • 资助金额:
    58.0万元
  • 批准年份:
    2019
  • 负责人:
    赵阳
  • 依托单位:
棉花TERMINAL FLOWER1亚家族基因调控开花转变与株型发育的分子机制
  • 批准号:
    31860393
  • 项目类别:
    地区科学基金项目
  • 资助金额:
    39.0万元
  • 批准年份:
    2018
  • 负责人:
    黄先忠
  • 依托单位: