Functions of Auxin Response Factors

生长素反应因子的功能

• 批准号: 0116106
• 负责人: Jason Reed
• 金额: $ 29.43万
• 依托单位: University of North Carolina at Chapel Hill
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2004-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0116106&HistoricalAwards=false
• 关键词: Functions; Auxin; Response; Factors

0116106ReedThe plant hormone auxin controls multiple developmental processes including elongation of stems, branching of shoots and roots, bending toward or away from light or gravity, and patterning of various tissues. Auxin Response Factors (ARFs) are a family transcription factors that regulate induction and repression of genes in response to auxin, and therefore have key functions in mediating these developmental processes. This project will focus on functions and interactions of ARF6 and ARF8, two ARFs in the model plant Arabidopsis thaliana that activate genes in response to auxin. The aims of the project are i) to discover developmental functions of these two transcription factors by characterizing phenotypes of arf6 and arf8 mutant plants and of transgenic plants that overexpress epitope-tagged ARF6 or ARF8; ii) to identify genes that ARF6 and ARF8 regulate by hybridizing probes made from untreated or auxin-treated wild-type and mutant plants to gene chips containing DNA representing thousands of Arabidopsis genes; iii) to explore functional interactions between ARF6 and ARF8 and selected proteins of the related Aux/IAA family, by constructing double mutants between arf6 and arf8 and selected mutations in IAA genes and examining their phenotypes; iv) to raise ARF6- and ARF8-specific anti-peptide antibodies; and v) to use these antibodies and the epitope-tagged proteins to study whether ARF6 and ARF8 interact with other proteins in vivo. These studies will provide a multifaceted view of the functions of ARF6 and ARF8, setting the stage for similar analyses of other

[01:16 . 06]植物激素生长素控制着多种发育过程，包括茎的伸长、茎和根的分支、对光或重力的弯曲和各种组织的形成。生长素反应因子（Auxin Response Factors, ARFs）是一种调节生长素诱导和抑制基因的家族转录因子，因此在介导这些发育过程中具有关键作用。本项目将重点研究模式植物拟南芥中ARF6和ARF8的功能和相互作用，这两个ARF6和ARF8在生长素响应中激活基因。该项目的目的是：i)通过表征arf6和arf8突变植物以及过表达表位标记的arf6或arf8的转基因植物的表型，发现这两种转录因子的发育功能；ii)通过将未经处理或生长素处理的野生型和突变型植物制成的探针与含有代表数千个拟南芥基因的DNA的基因芯片杂交，鉴定ARF6和ARF8调控的基因；iii)通过构建ARF6和ARF8与选定的IAA基因突变之间的双突变体并检测其表型，探索ARF6和ARF8与Aux/IAA相关家族选定蛋白之间的功能相互作用；iv)培养ARF6-和arf8特异性抗肽抗体；v)利用这些抗体和表位标记蛋白在体内研究ARF6和ARF8是否与其他蛋白相互作用。这些研究将对ARF6和ARF8的功能提供一个多方面的观点，为其他类似的分析奠定基础