Biophotonics: Collaborative Research: Photoactivated Coupling of Nanoparticle Multilayers and Nerve Cells

[194883]最近纳米技术和生物工程的进展为促进电子、光学和生物系统的直接相互作用开辟了令人兴奋的前景。在神经科学和医疗器械（如假肢）领域实现这一目标的主要障碍是对活神经元与纳米材料之间耦合的分子过程的理解不足，以及如何优化人造材料与生命系统之间的耦合。在这个项目中，目的是研究由纳米颗粒和培养的神经细胞组成的薄膜模型系统中活/无生命物质相互作用的动力学和机制。多学科和多机构的“关联合作提案”汇集了几个具有广泛专业知识和研究兴趣的研究小组，进行实验和理论研究，旨在描述纳米材料和神经元界面上发生的相互作用，并优化界面，以便在光子探测附着在细胞上的纳米材料界面后有效地激活神经元。由Kotov博士领导的俄克拉荷马州立大学的合作伙伴将开展该项目的材料科学方面，而由Motamedi博士领导的德克萨斯大学医学分部的调查将集中在这项工作的生物工程和电生理组成部分。具体来说，项目的目标如下。(1)制备可附着于神经细胞的生物相容性纳米颗粒多层膜。(2)光诱导神经细胞膜的电流和电位在界面光激发下作为NP和生物结构的功能的登记和表征。(3)不同界面结构下NP-cell耦合优化。

0119483KotovRecent advances in nanotechnology and bioengineering have opened the exciting prospect for promoting direct interaction of electronics, optics and biological systems. The primary obstacle to realizing this goal in the fields of neuroscience and medical devices such as prostheses is the inadequate understanding of the molecular processes involved in coupling between living neurons and nanomaterials, and how to optimize the coupling between man made materials and living systems. In this project, the intent is to investigate the dynamics and mechanisms of the live/lifeless matter interaction in a model system consisting of a thin film composed of nanoparticles and cultured nerve cells. The multi-disciplinary and multi-institutional "Linked Collaborative Proposals " bring together several research groups with broad expertise and research interest to conduct experimental and theoretical studies aimed at characterizing the interaction that occur at the interface of nanomaterials and neurons and optimizing the interface for effective photon-activation of neurons following photonic probing of the interface of nanomaterials that are attached to the cells. The partners at Oklahoma State University lead by Dr. Kotov will carry out the materials science aspect of this project, while the investigation at the University of Texas Medical Branch lead by Dr. Motamedi will concentrate on the bioengineering and electrophysiological components of this work. Specifically, the objectives of the proje ct are the following. (1) Preparation of biocompatible nanoparticle multilayers that can be attached to nerve cells. (2) Registration and characterization of the photoinduced nerve cell membrane currents and potentials following optical excitation of the interface as function of NP and biological structures. (3) Optimization of NP-cell coupling for different interface structure.