Biophotonics: Electrochemiluminescence at Microelectrodes During PCR Amplification of DNA

生物光子学：DNA PCR 扩增过程中微电极的电化学发光

• 批准号: 0352401
• 负责人: Rosemary Smith
• 金额: $ 28.6万
• 依托单位: University of Maine
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0352401&HistoricalAwards=false
• 关键词: Biophotonics; Electrochemiluminescence; Microelectrodes; During; PCR

0086863SmithThis project investigates a new technique for in situ quantification of Polymerase Chain Reaction (PCR) amplification products using electrochemiluminescence (ECL). The technique implements the highly sensitive, ECL detection and quantification of tris(2,2'bipyridyl) ruthenium (II) labeled DNA. This method is expected to yield significant improvement in speed, cost and performance over existing quantitative PCR methods, by reducing the number and quantity of reagents, reducing the number of sample preparation steps, increasing sensitivity, and shortening analysis time.

0086863史密斯本项目研究了一种新的技术，用于使用电化学发光（ECL）原位定量聚合酶链反应（PCR）扩增产物。 该技术实现了三（2，2 '联吡啶）钌（II）标记的DNA的高灵敏度，ECL检测和定量。 与现有的定量PCR方法相比，该方法预计将在速度、成本和性能方面取得显着改善，减少试剂的数量和数量，减少样本制备步骤的数量，提高灵敏度并缩短分析时间。