Biophotonics: Electrochemiluminescence at Microelectrodes During PCR Amplification of DNA

本项目研究了一种利用电化学发光（ECL）原位定量聚合酶链反应（PCR）扩增产物的新技术。该技术实现了三（2,2'联吡啶基）钌（II）标记DNA的高灵敏度ECL检测和定量。该方法减少了试剂的数量和数量，减少了样品制备步骤，提高了灵敏度，缩短了分析时间，与现有的定量PCR方法相比，有望在速度、成本和性能方面取得显著改善。

0086863SmithThis project investigates a new technique for in situ quantification of Polymerase Chain Reaction (PCR) amplification products using electrochemiluminescence (ECL). The technique implements the highly sensitive, ECL detection and quantification of tris(2,2'bipyridyl) ruthenium (II) labeled DNA. This method is expected to yield significant improvement in speed, cost and performance over existing quantitative PCR methods, by reducing the number and quantity of reagents, reducing the number of sample preparation steps, increasing sensitivity, and shortening analysis time.