A Confocal Microscope for 4-Dimensional Biological Imaging

这笔拨款为获得具有光谱成像能力的共聚焦激光扫描显微镜（CLSM）提供了部分支持。该奖项在凯文·爱德华兹博士的指导下授予伊利诺伊州立大学（ISU）。该显微镜将被许多研究人员用于细胞和发育生物学的各种研究项目，包括：(1)保守的酪氨酸磷酸酶在细胞信号传导和发育中的作用，(2)锚蛋白突变对线虫神经发育的影响，(3)控制植物细胞定向生长的分子过程的遗传解剖，(4)基于微探针的新型细胞成像技术的发展，(5)大鼠帕金森病模型脑组织中多巴胺神经元的分布，(6)植物细胞分裂进化的研究，(7)离子转运蛋白在鱼类适应渗透变化中的作用；(8)昆虫繁殖过程中卵泡细胞形态变化的内分泌调节研究。共聚焦显微镜将成为多用户设施的一部分，并将加强促进国际滑联几个关键任务所需的基础设施：增加本科生的研究保留，增强科学多样性，加强K-12科学教学。ISU为大量以前可能很少接触当前科学工具的学生提供服务。此外，该仪器将成为伊利诺伊卫斯理大学、米利金大学、诺克斯学院、伊利诺伊大学医学院-皮奥里亚分校和皮奥里亚USDA-ARS设施最近的共聚焦显微镜，从而为这些机构提供新的研究和培训机会。

This grant provides partial support for the acquisition of a confocal laser scanning microscope (CLSM) with spectral imaging capabilities. The award is made to Illinois State University (ISU) under the direction of Dr. Kevin Edwards. The microscope will be employed by a number of investigators for diverse research projects in cell and developmental biology, including: (1) the roles of conserved tyrosine phosphatases in cell signaling and development, (2) the effects of an ankyrin mutation on C. elegans neural development, (3) genetic dissection of the molecular processes that control directional growth of plant cells, (4) the development of novel microprobe-based cell imaging techniques, (5) the distribution of dopamine neurons in brain tissue in a rat Parkinson Disease model, (6) the investigation of the evolution of cell division in plants, (7) the role of ion transport proteins in the adaptation of fish to osmotic changes, and (8) the investigation of the endocrine regulation of follicle cell shape changes during insect reproduction. The confocal microscope will be part of a multi-user facility, and will strengthen the infrastructure required to promote several of ISU's key missions: increased retention of undergraduates in research, enhancement of diversity in science, and strengthening K-12 science teaching. ISU serves a large pool of students who may have had little previous exposure to current scientific tools. Also, this instrument will be the nearest confocal microscope for Illinois Wesleyan Univ., Millikin Univ, Knox College, University of Illinois College of Medicine-Peoria, and the Peoria USDA-ARS facility, and thus will provide new research and training opportunities for those institutions.