A Confocal Microscope for 4-Dimensional Biological Imaging

用于 4 维生物成像的共焦显微镜

• 批准号: 0400675
• 负责人: Kevin Edwards
• 金额: $ 20.77万
• 依托单位: Board of Trustees of Illinois State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0400675&HistoricalAwards=false
• 关键词: Confocal; Microscope; Dimensional; Biological; Imaging

This grant provides partial support for the acquisition of a confocal laser scanning microscope (CLSM) with spectral imaging capabilities. The award is made to Illinois State University (ISU) under the direction of Dr. Kevin Edwards. The microscope will be employed by a number of investigators for diverse research projects in cell and developmental biology, including: (1) the roles of conserved tyrosine phosphatases in cell signaling and development, (2) the effects of an ankyrin mutation on C. elegans neural development, (3) genetic dissection of the molecular processes that control directional growth of plant cells, (4) the development of novel microprobe-based cell imaging techniques, (5) the distribution of dopamine neurons in brain tissue in a rat Parkinson Disease model, (6) the investigation of the evolution of cell division in plants, (7) the role of ion transport proteins in the adaptation of fish to osmotic changes, and (8) the investigation of the endocrine regulation of follicle cell shape changes during insect reproduction. The confocal microscope will be part of a multi-user facility, and will strengthen the infrastructure required to promote several of ISU's key missions: increased retention of undergraduates in research, enhancement of diversity in science, and strengthening K-12 science teaching. ISU serves a large pool of students who may have had little previous exposure to current scientific tools. Also, this instrument will be the nearest confocal microscope for Illinois Wesleyan Univ., Millikin Univ, Knox College, University of Illinois College of Medicine-Peoria, and the Peoria USDA-ARS facility, and thus will provide new research and training opportunities for those institutions.

这笔赠款为购买具有光谱成像能力的共焦激光扫描显微镜(CLSM)提供了部分支持。该奖项在凯文·爱德华兹博士的指导下颁发给伊利诺伊州立大学(ISU)。该显微镜将被许多研究人员用于细胞和发育生物学的不同研究项目，包括：(1)保守的酪氨酸磷酸酶在细胞信号和发育中的作用，(2)Ankyrin突变对线虫神经发育的影响，(3)控制植物细胞定向生长的分子过程的遗传解剖，(4)基于微探针的新细胞成像技术的发展，(5)大鼠帕金森病模型脑组织中多巴胺神经元的分布，(6)植物细胞分裂的进化，(7)离子转运蛋白在鱼类适应渗透变化中的作用；(8)昆虫繁殖过程中毛囊细胞形状变化的内分泌调节。共焦显微镜将是多用户设施的一部分，并将加强所需的基础设施，以促进执行支助股的几项主要任务：增加本科生在研究中的留存，增强科学的多样性，以及加强K-12科学教学。ISU为一大批学生提供服务，这些学生以前可能几乎没有接触过当前的科学工具。此外，该仪器将是伊利诺伊州卫斯理大学、密利金大学、诺克斯学院、伊利诺伊大学皮奥里亚医学院和皮奥里亚USDA-ARS设施最近的共焦显微镜，因此将为这些机构提供新的研究和培训机会。