MRI: Acquisition of a Focused Ion Beam Scanning Electron Microscope (FIB-SEM) for Large Volume 3 Dimensional Imaging of Whole Cells

耶鲁大学获得了一项奖励，以实现最先进的聚焦离子束扫描电子显微镜（FIB-SEM）系统。它提供了一个强大的新工具来研究细胞及其细胞器的三维结构和功能-特别是那些需要高分辨率，但大体积视图的。它也将允许新的相关研究，弥合光学显微镜（包括超分辨率）和电子显微镜之间的差距。更广泛的影响是多方面的。该仪器将集成到耶鲁大学CCMI电子显微镜核心设施和为这种多用户设备创建的基础设施中。该仪器将用于培养定量成像的下一代科学家和生物工程师，促进生物、工程和物理科学之间新的跨学科研究。学生多样性是一个非常重要的重点，并将通过耶鲁建立的外展项目在多个方面积极促进。3D EM数据集将在立体虚拟现实（VR）平台上呈现，因此任何人都可以进入细胞。因此，大量的高分辨率体积数据不仅将推动基础科学，而且有助于教育和激励广泛而多样化的受众。新的FIB-SEM系统将为细胞和组织结构如何影响功能提供新的科学研究，包括神经元如何沟通，初级纤毛、高尔基复合体、吞噬体和内质网等细胞器如何形成和功能。主要用户群包括生物科学、工程和计算机科学等广泛科学学科的领导者。新系统将作为一个焦点，教员、博士后学员和来自不同部门的学生相互交流，互相帮助，以达到共同的成像和分析目标。该仪器将是一个非常有价值的工具，不仅对细胞内功能的研究，而且对研究细胞间通讯的项目，如发生在神经系统和免疫系统中的通讯。

An award is made to Yale University to implement a state-of-the-art Focused Ion Beam Scanning Electron Microscope (FIB-SEM) system. It provides a powerful new tool to study the 3D structure and function of cells and their organelles - especially those that require high-resolution, yet large volumetric views. It will also allow new correlative studies that bridge the gap between light microscopy (including super-resolution) and electron microscopy. The broader impacts are multifaceted. The instrument will be integrated into the Yale CCMI Electron Microscopy Core Facility and infrastructure created precisely for such multi-user equipment. The instrument will be used to train the next generation of scientists and bioengineers in quantitative imaging, fostering new interdisciplinary research between biological, engineering and physical sciences. Student diversity is a strong focus and will be actively promoted on multiple fronts through established Yale outreach programs. 3D EM datasets will be rendered in a stereoscopic virtual reality (VR) platform so that anyone can voyage into cells. Thus, the large high-resolution volumetric data will not only propel basic science, but also help educate and inspire a broad and diverse audience. The new FIB-SEM system will enable new scientific research into how cell and tissue structure impacts function, with projects on how neurons communicate, how organelles such as primary cilia, Golgi complex, phagosomes, and the endoplasmic reticulum form and function. The main user group comprises leaders in a broad range of scientific disciplines stretching across the biological sciences, engineering, and computer sciences. The new system will act as a focal point, with faculty members, postdoctoral trainees, and students from different departments interacting and helping each other to reach common imaging and analysis goals. The instrument will be an extremely valuable tool not only for research on intracellular functions, but also for projects that address intercellular communications, such as those that occur in the nervous system and in the immune system.