Instrumentation for High-Throughput Genotyping, Fingerprinting, and Sequencing Projects

这项奖励为购买用于植物和微生物分析的几种仪器提供支持。该设备包括一台16毛细管DNA测序仪、PCR机、一个液体机器人处理机和必要的软件。该仪器增加了伍斯特俄亥俄州立大学校区和附近希拉姆学院的几个正在进行的研究项目所需的吞吐能力。这些仪器将放置在分子和细胞成像中心(MCIC)，这是校园内一个多学科的核心设施。预计将使用这些仪器的研究项目包括番茄果实形态变异的研究，卵菌真菌与植物寄主相互作用的分子基础研究，植物抗病和非生物胁迫数量变异的分子研究，以及微生物基因组的高通量基因分型和测序。除了其在研究方面的作用外，这些设备还将被纳入一系列正在进行的外联和培训活动中，目前该中心是这些活动的协调中心。来自十个不同部门的28个实验室的研究生、博士后研究员、教师和技术人员使用该设施进行与他们的论文工作或研究相关的研究。此外，来自私立、州立、社区和少数民族大学的本科生参加伍斯特校园的暑期研究项目。这些学生将有机会获得所需仪器的培训和使用。MCIC经常为高中生和教师、K-12学生以及该地区的居民举办参观活动，以更好地了解植物、动物和微生物研究，并让更广泛的社区参与与科学相关的活动。

This award provides support for purchase of several instruments used in analysis of plants and microbes. The equipment includes a 16 capillary DNA sequencer, PCR machines, a liquid robotic handler, and necessary software. The instrumentation has the increased throughput capacity needed for several ongoing research projects of faculty and students at the OSU campus in Wooster, and at nearby Hiram College. The instruments will be placed in the Molecular and Cellular Imaging Center (MCIC), a multi-disciplinary core facility on the campus. Research programs expected to use the instruments include studies of variation in tomato fruit morphology, studies of the molecular basis of interactions between oomycete fungi and their plant hosts, molecular studies of quantitative variation in plant resistance to disease and abiotic stress, and high throughput genotyping and sequencing of microbial genomes. In addition to its role in research, the equipment will be integrated into a broad range of ongoing outreach and training activities for which the MCIC is currently a focal point. Graduate students, postdoctoral fellows, faculty and technical staff from more than 28 laboratories in ten different departments use the facility for studies related to their thesis work or research. In addition, undergraduate students from private, state, community, and minority colleges participate in summer research programs on the Wooster campus . These students will have access to training and use of the requested instrumentation. The MCIC hosts frequent tours for high school students and teachers, K-12 students, as well as area residents to provide a greater understanding of plant, animal and microbial research and to engage the broader community in science-related activities .