Instrumentation for High-Throughput Genotyping, Fingerprinting, and Sequencing Projects

该奖项为购买几种用于分析植物和微生物的仪器提供支持。设备包括16毛细管DNA测序仪，PCR仪，液体机器人处理器和必要的软件。该仪器具有增加的吞吐量，以满足俄勒冈州立大学伍斯特校区和附近的海勒姆学院的教师和学生正在进行的几个研究项目的需要。这些仪器将被放置在分子和细胞成像中心（MCIC），这是校园内的一个多学科核心设施。预计将使用这些仪器的研究项目包括番茄果实形态变异的研究、卵菌真菌与其植物宿主相互作用的分子基础研究、植物抗病和非生物胁迫的定量变异的分子研究，以及微生物基因组的高通量基因分型和测序。除了在研究方面的作用外，这些设备还将被纳入正在进行的广泛的外联和培训活动中，目前该中心是这些活动的一个协调中心。来自10个不同院系28个以上实验室的研究生、博士后、教职员工和技术人员使用该设施进行与他们的论文工作或研究相关的研究。此外,大学生从私人、州社区和少数民族院校参与夏季研究项目在伍斯特校园。这些学生将有机会接受培训并使用所要求的仪器。MCIC经常为高中学生和教师，K-12学生以及地区居民举办参观活动，以提供对植物，动物和微生物研究的更多了解，并使更广泛的社区参与与科学相关的活动。

This award provides support for purchase of several instruments used in analysis of plants and microbes. The equipment includes a 16 capillary DNA sequencer, PCR machines, a liquid robotic handler, and necessary software. The instrumentation has the increased throughput capacity needed for several ongoing research projects of faculty and students at the OSU campus in Wooster, and at nearby Hiram College. The instruments will be placed in the Molecular and Cellular Imaging Center (MCIC), a multi-disciplinary core facility on the campus. Research programs expected to use the instruments include studies of variation in tomato fruit morphology, studies of the molecular basis of interactions between oomycete fungi and their plant hosts, molecular studies of quantitative variation in plant resistance to disease and abiotic stress, and high throughput genotyping and sequencing of microbial genomes. In addition to its role in research, the equipment will be integrated into a broad range of ongoing outreach and training activities for which the MCIC is currently a focal point. Graduate students, postdoctoral fellows, faculty and technical staff from more than 28 laboratories in ten different departments use the facility for studies related to their thesis work or research. In addition, undergraduate students from private, state, community, and minority colleges participate in summer research programs on the Wooster campus . These students will have access to training and use of the requested instrumentation. The MCIC hosts frequent tours for high school students and teachers, K-12 students, as well as area residents to provide a greater understanding of plant, animal and microbial research and to engage the broader community in science-related activities .