Instrumentation for High-Throughput Genotyping, Fingerprinting, and Sequencing Projects

高通量基因分型、指纹图谱和测序项目的仪器

• 批准号: 0400811
• 负责人: Esther Van der Knaap
• 金额: $ 19.92万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0400811&HistoricalAwards=false
• 关键词: Instrumentation; Throughput; Genotyping; Fingerprinting; Sequencing

This award provides support for purchase of several instruments used in analysis of plants and microbes. The equipment includes a 16 capillary DNA sequencer, PCR machines, a liquid robotic handler, and necessary software. The instrumentation has the increased throughput capacity needed for several ongoing research projects of faculty and students at the OSU campus in Wooster, and at nearby Hiram College. The instruments will be placed in the Molecular and Cellular Imaging Center (MCIC), a multi-disciplinary core facility on the campus. Research programs expected to use the instruments include studies of variation in tomato fruit morphology, studies of the molecular basis of interactions between oomycete fungi and their plant hosts, molecular studies of quantitative variation in plant resistance to disease and abiotic stress, and high throughput genotyping and sequencing of microbial genomes. In addition to its role in research, the equipment will be integrated into a broad range of ongoing outreach and training activities for which the MCIC is currently a focal point. Graduate students, postdoctoral fellows, faculty and technical staff from more than 28 laboratories in ten different departments use the facility for studies related to their thesis work or research. In addition, undergraduate students from private, state, community, and minority colleges participate in summer research programs on the Wooster campus . These students will have access to training and use of the requested instrumentation. The MCIC hosts frequent tours for high school students and teachers, K-12 students, as well as area residents to provide a greater understanding of plant, animal and microbial research and to engage the broader community in science-related activities .

该奖项为购买用于植物和微生物分析的几种仪器提供支持。该设备包括一个16毛细管DNA测序仪，PCR机，液体机器人处理器，和必要的软件。 仪器具有增加的吞吐量所需的几个正在进行的研究项目的教师和学生在俄勒冈州立大学校园伍斯特，并在附近的希拉姆学院。 这些仪器将被放置在分子和细胞成像中心（MCIC），这是校园内的一个多学科核心设施。 预计使用这些仪器的研究项目包括番茄果实形态变异的研究、卵菌纲真菌与其植物宿主之间相互作用的分子基础的研究、植物抗病性和非生物胁迫的定量变异的分子研究以及高通量基因分型和微生物基因组测序。 这些设备除了在研究方面发挥作用外，还将被纳入正在进行的广泛的外联和培训活动，而军事和信息中心目前是这些活动的协调中心。 来自10个不同部门的28个实验室的研究生，博士后研究员，教师和技术人员使用该设施进行与论文工作或研究相关的研究。 此外，来自私立，州立，社区和少数民族学院的本科生参加伍斯特校园的夏季研究项目。 这些学生将有机会获得培训和使用所要求的仪器。 MCIC经常为高中学生和教师、K-12学生以及当地居民举办图尔斯之旅，以更好地了解植物、动物和微生物研究，并让更广泛的社区参与科学相关活动。