A DNA Sequencer, Real-time PCR Machine, Gradient Thermal Cycler, and Auxiliary Equipment for Research and Training in Molecular Evolution and Ecology

用于分子进化和生态学研究和培训的DNA测序仪、实时PCR仪、梯度热循环仪和辅助设备

• 批准号: 0400829
• 负责人: John True
• 金额: $ 15.91万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0400829&HistoricalAwards=false
• 关键词: DNA; Sequencer; Real; time; PCR

This award provides partial support for the purchase of a 16-capillary DNA sequencer, real-time PCR machine, gradient PCR thermal cycler, and data analysis system - all to be housed in a shared facility. The instrumentation will be used in studies of molecular-level variation in nature, and the evolutionary implications of that variation. Several capabilities that are crucial to current evolutionary and ecological research programs will be developed, including: (1) analysis of highly polymorphic genotypes (microsatellite, SNP, AFLP, TRFLP); (2) quantitative PCR analysis of gene expression; and (3) DNA sequencing. The six major users of the instrumentation represent a diverse set of research programs in which understanding the basis of biological diversity, through the use of these molecular tools, is a principal goal. In addition, the inclusion of a sample preparation facility and center for computational analysis of molecular data will enable many other Stony Brook research groups to address important ecological and evolutionary questions with novel forms of data. Establishment of this central laboratory for molecular studies of adaptation and diversity will also have significant broader impacts on life sciences research and teaching at Stony Brook and in the larger community. First, the instrumentation will substantially augment the training capabilities of our department and enable new interactions and collaborations among biologists throughout the Stony Brook community. Additionally, the laboratory will be used for undergraduate training in basic molecular biology techniques, vital to many careers in the life sciences. This represents a novel and tangible benefit to the Stony Brook undergraduate community, which is one the most diverse in the nation. Finally, by synergizing with the activities of the Long Island Group for Advancing Science Education (LIGASE), the proposed facility will augment outreach to local K-12 students and educators, representing a new and important means for presenting ecological and evolutionary ideas and information to the public at large.

该合同为购买16毛细管DNA测序仪、实时PCR仪、梯度PCR热循环仪和数据分析系统提供部分支持-所有这些都将被安置在一个共享设施中。 该仪器将用于自然界分子水平变化的研究，以及这种变化的进化意义。将开发对当前进化和生态研究计划至关重要的几种能力，包括：（1）高度多态性基因型（微卫星，SNP，AFLP，TRFLP）分析;（2）基因表达的定量PCR分析;和（3）DNA测序。仪器的六个主要用户代表了一套不同的研究方案，其中通过使用这些分子工具了解生物多样性的基础是一个主要目标。 此外，包括一个样品制备设施和中心的分子数据的计算分析将使许多其他斯托尼布鲁克研究小组，以解决重要的生态和进化问题的新形式的数据。 建立这一适应性和多样性分子研究中心实验室也将对斯托尼布鲁克和更大社区的生命科学研究和教学产生更广泛的影响。 首先，仪器将大大增强我们部门的培训能力，并使整个斯托尼布鲁克社区的生物学家之间的新的互动和合作。此外，该实验室将用于基础分子生物学技术的本科生培训，这对生命科学的许多职业至关重要。 这对斯托尼布鲁克的本科生社区来说是一个新颖而切实的好处，它是全国最多样化的大学之一。 最后，通过与长岛推进科学教育集团（LIGASE）的活动协同作用，拟议的设施将扩大对当地K-12学生和教育工作者的宣传，代表向公众展示生态和进化思想和信息的新的重要手段。