Bal-Tec HPM010 High Pressure Freezer

Bal-Tec HPM010 高压冷冻机

• 批准号: 0400874
• 负责人: Erik Jorgensen
• 金额: $ 15.42万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0400874&HistoricalAwards=false
• 关键词: Bal; Tec; HPM010; Pressure; Freezer

This award provides partial support for purchase of a device used for rapid freezing of samples for electron microscopy. Following fixation at low temperature, cells preserved through use of high pressure freezing have improved ultrastructure and are suitable for immunocytochemistry. Use of high pressure permits the freezing of thicker samples than alternative procedures, and is thus useful for reliable freezing of whole organisms with cuticles, such as nematodes and flies, and for freezing of yeast and plant cells that have thick cell walls. The instrument will be used for study of synaptic ultrastructure in nematode mutants, protein localization at membranes of muscles and neurons, ultrastructure of yeast mitochondria, and developmental defects in plant mutants. The equipment will be housed in an imaging facility at the University of Utah where it will be available for other research and training use by students and faculty from the University and from throughout the Great Basin area.

该奖项为购买用于快速冷冻电子显微镜样品的设备提供部分支持。 在低温下固定后，通过使用高压冷冻保存的细胞具有改善的超微结构，并且适合于免疫细胞化学。 使用高压允许冷冻比替代程序更厚的样品，并且因此可用于可靠地冷冻具有微生物的整个生物体，例如线虫和蝇，以及用于冷冻具有厚细胞壁的酵母和植物细胞。 该仪器将用于研究线虫突变体的突触超微结构、肌肉和神经元膜上的蛋白质定位、酵母线粒体的超微结构以及植物突变体的发育缺陷。 这些设备将被安置在犹他州大学的一个成像设施中，供该大学和整个大盆地地区的学生和教师进行其他研究和培训。