Bal-Tec HPM010 High Pressure Freezer

该奖项为购买用于快速冷冻电子显微镜样品的设备提供部分支持。 在低温下固定后，通过使用高压冷冻保存的细胞具有改善的超微结构，并且适合于免疫细胞化学。 与替代程序相比，使用高压可以冷冻更厚的样本，因此对于可靠冷冻具有角质层的整个生物体（例如线虫和苍蝇）以及冷冻具有厚细胞壁的酵母和植物细胞非常有用。 该仪器将用于研究线虫突变体的突触超微结构、肌肉和神经元膜上的蛋白质定位、酵母线粒体的超微结构以及植物突变体的发育缺陷。 这些设备将被安置在犹他州大学的一个成像设施中，供该大学和整个大盆地地区的学生和教师进行其他研究和培训。

This award provides partial support for purchase of a device used for rapid freezing of samples for electron microscopy. Following fixation at low temperature, cells preserved through use of high pressure freezing have improved ultrastructure and are suitable for immunocytochemistry. Use of high pressure permits the freezing of thicker samples than alternative procedures, and is thus useful for reliable freezing of whole organisms with cuticles, such as nematodes and flies, and for freezing of yeast and plant cells that have thick cell walls. The instrument will be used for study of synaptic ultrastructure in nematode mutants, protein localization at membranes of muscles and neurons, ultrastructure of yeast mitochondria, and developmental defects in plant mutants. The equipment will be housed in an imaging facility at the University of Utah where it will be available for other research and training use by students and faculty from the University and from throughout the Great Basin area.