NIRT: DNA Sequencing and Translocation Studies using Electrically-Addressable Nanopore Arrays

NIRT：使用电可寻址纳米孔阵列进行 DNA 测序和易位研究

• 批准号: 0403891
• 负责人: Xinsheng Ling
• 金额: --
• 依托单位: Brown University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0403891&HistoricalAwards=false
• 关键词: NIRT; DNA; Sequencing; Translocation; Studies

This proposal was received in response to Nanoscale Science and Engineering initiative, NSF 03-043, category NIRT. The research program will utilize the complimentary expertise of the PI and co-PIs from different disciplines to develop a new generation of solid-state nanopore device that will allow measurements with unprecedented accuracies, and exploration of new DNA sequencing mechanisms. The NIRT team will: (1) Develop an electrically-addressable nanopore array (EANA) device and its related microfluid systems. (2) Develop a hybrid nanopore-in-nanopore system using EANA and biological nanopores, and carry out the first multiplexing Hemolysin nanopore experiments; (3) Explore the feasibility of sequence detection using SiO2 nanopores functionalized with polypeptide nucleic acid (PNA) molecules in the pore region; (4) Carry out an experimental test of a theoretical model proposed by a co-PI using single strand binding proteins to drive DNA translocation, and to examine its implications for sequencing; (5) Carry out a feasibility study of using the EANA technology for real-time monitoring of DNA degradation processes at single molecule level, and its potential for DNA sequencing. These NIRT activities will have broad impact in biological and biomedical research, in providing new techniques and more efficient analytical tools for studying biomolecules. The projects provide excellent training opportunities for postdoctoral and graduate students at the forefront of nanoscience and single-molecule biophysics and theoretical physics. The program also offers undergraduate students at Brown University and Harvard University new exciting research experience in the interdisciplinary field of nanobioscience. The students will receive advanced training in silicon nanofabrication, nano- and microfluidics, optics, molecular biophysics, and many-body statistical physics. These new skills will prepare the students for future careers in industry, academia, and public service. Funds for this award are provided by the Divisions of Physics and Materials Science in the Mathematical and Physical Sciences Directorate, the Division of Engineering Education and Centers in the Engineering Directorate, and the Division of Biological Infrastructure in the Directorate for Biological Sciences.

该提案是响应纳米科学与工程倡议，NSF 03-043，类别NIRT。该研究计划将利用来自不同学科的PI和co-PI的互补专业知识来开发新一代固态纳米孔设备，该设备将允许以前所未有的精度进行测量，并探索新的DNA测序机制。NIRT团队将：（1）开发电寻址纳米孔阵列（EANA）设备及其相关的微流体系统。(2)利用EANA和生物纳米孔构建了纳米孔中纳米孔的混合体系，并首次开展了溶血素纳米孔的多重实验;（3）探索了利用孔区多肽核酸（PNA）分子功能化的SiO2纳米孔进行序列检测的可行性;（4）对co-PI提出的使用单链结合蛋白驱动DNA易位的理论模型进行实验测试，（5）开展EANA技术在单分子水平上实时监测DNA降解过程的可行性研究及其在DNA测序中的应用潜力。这些NIRT活动将在生物和生物医学研究中产生广泛的影响，为研究生物分子提供新技术和更有效的分析工具。这些项目为纳米科学和单分子生物物理学和理论物理学前沿的博士后和研究生提供了极好的培训机会。该计划还为布朗大学和哈佛大学的本科生提供了纳米生物科学跨学科领域的新的令人兴奋的研究经验。学生将接受硅纳米纤维，纳米和微流体，光学，分子生物物理学和多体统计物理学的高级培训。这些新技能将为学生未来在工业，学术界和公共服务领域的职业生涯做好准备。该奖项的资金由数学和物理科学理事会的物理和材料科学部门，工程教育和工程理事会中心部门以及生物科学理事会生物基础设施部门提供。