NER: DNA Sequence Detection Using Novel Solid-State and Soft Nanopores

NER：使用新型固态和软纳米孔进行 DNA 序列检测

• 批准号: 0304325
• 负责人: Xinsheng Ling
• 金额: $ 10万
• 依托单位: Brown University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0304325&HistoricalAwards=false
• 关键词: NER; DNA; Sequence; Detection; Using

This Nanoscale Exploratory Research (NER) proposal was received in response to NSE, NSF 02-148. The one-year project will develop novel solid-state and soft nanopore devices for rapid DNA sequencing. It has been suggested that one may be able to detect genetic sequences in individual DNA molecules by measuring the change in the ionic conductance of a nanometer-scaled pore embedded in an insulating membrane as a single-stranded ssDNA molecule is driven through by an electric field. The use of two types of new synthetic nanopores will be explored: (1) a nanopore with 1 nm in length or depth fabricated by molding soft materials using a novel "cutting-edges" concept; (2) an integrated nanopore circuit consisting of three nanopores in series using silicon technology. Novel experiments will be performed to study the microscopic processes by which a long ssDNA molecule "translocates" through a nanopore, and to explore a new sensing mechanism exploiting the hydrogen-bonding effects between two ssDNA molecules. The proposed projects, if successful, could lead to a great advance in the development of a nanopore DNA sequencing technology. The projects will provide research opportunities for graduate and undergraduate students in an exciting emerging field of single molecule biophysics. The students will receive advanced training in the state-of-the-art nanofabrication technology and single-molecule biophysics. This project is jointly supported by the Divisions of Materials Research and Physics in the Directorate for Mathematical and Physical Sciences, as well as the Division of Emerging Frontiers in the Directorate for Biological SciencesThis Nanoscale Exploratory Research (NER) proposal was received in response to NSE, NSF 02-148. The one-year project will explore a new generation of nanometer-scaled sensors, solid-state nanopores, for detecting and characterization of biological molecules. Nanopore biosensors have shown great promise in basic studies of single-molecule biophysics. These novel devices may also be useful in disease control and in anti-bioterrorism. Two types of new synthetic nanopores will be explored: one based on the state-of-the-art silicon technology, and the other based on polymeric materials. The proposed projects, if successful, could lead to a great advance in the development of a nanopore DNA sequencing technology. The project will provide research opportunities for graduate and undergraduate students in an exciting emerging field of single molecule biophysics. The students will receive advanced training in the state-of-the-art nanofabrication technology and single-molecule biophysics. This project is jointly supported by the Divisions of Materials Research and Physics in the Directorate for Mathematical and Physical Sciences, as well as the Division of Emerging Frontiers in the Directorate for Biological Sciences

本纳米尺度探索性研究（NER）提案已收到NSE， NSF 02-148的响应。这个为期一年的项目将开发用于快速DNA测序的新型固态和软纳米孔设备。有人建议，当单链ssDNA分子被电场驱动通过时，可以通过测量嵌入绝缘膜中的纳米级孔的离子电导变化来检测单个DNA分子中的基因序列。将探索两种新型合成纳米孔的使用：(1)采用新颖的“前沿”概念，通过模压软材料制造出长度或深度为1纳米的纳米孔；(2)采用硅技术制备由3个纳米孔串联而成的集成纳米孔电路。新的实验将用于研究长ssDNA分子通过纳米孔“易位”的微观过程，并探索利用两个ssDNA分子之间的氢键效应的新的传感机制。拟议的项目如果成功，可能会导致纳米孔DNA测序技术发展的巨大进步。这些项目将为研究生和本科生在单分子生物物理学这一令人兴奋的新兴领域提供研究机会。学生将接受先进的纳米制造技术和单分子生物物理学方面的高级培训。该项目由数学和物理科学理事会材料研究和物理部以及生物科学理事会新兴前沿部共同支持。该纳米尺度探索性研究（NER）提案已收到，响应NSE， NSF 02-148。这个为期一年的项目将探索新一代纳米级传感器，固态纳米孔，用于检测和表征生物分子。纳米孔生物传感器在单分子生物物理学的基础研究中显示出巨大的前景。这些新型装置也可用于疾病控制和反生物恐怖主义。将探索两种新型合成纳米孔：一种基于最先进的硅技术，另一种基于聚合物材料。拟议的项目如果成功，可能会导致纳米孔DNA测序技术发展的巨大进步。该项目将为研究生和本科生在单分子生物物理学这一令人兴奋的新兴领域提供研究机会。学生将接受先进的纳米制造技术和单分子生物物理学方面的高级培训。该项目由数学和物理科学理事会的材料研究和物理部门以及生物科学理事会的新兴前沿部门共同支持