NER: Nano-Scale Probe Array for Extraction of Genetic Material from Viruses

NER：用于从病毒中提取遗传物质的纳米级探针阵列

• 批准号: 0404107
• 负责人: Rashid Bashir
• 金额: $ 10万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-10-01 至 2005-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0404107&HistoricalAwards=false
• 关键词: NER; Nano; Scale; Probe; Array

Integrated biochips are promising devices for the rapid and accurate detection of biological entities such as cells, bacteria, spores, and viruses. These integrated biochips need to concentrate, trap, lyse, and perform genetic identification, all on the same device. Figure 1 shows an overall integrated biochip device that is being developed in our group. The accurate identification of these cells and microorganisms require the lysing of the cells to extract the DNA and proteins for molecular identification. DNA extraction using electrical means that are low power and integrated on to a microchip format can be very useful for realizing fully integrated bioanalytical devices. The objective of this project is to design and develop a method of extracting the genetic material from viruses within a handheld device which are low power, simple to use and compact. We will work with the Western Reserve Strain of Vaccinia Virus as the model virus as the main goal. As a stretch goal, we will perform the experiments with the Human Coronavirus

集成生物芯片是用于快速和准确检测生物实体（诸如细胞、细菌、孢子和病毒）的有前景的装置。这些集成的生物芯片需要集中，捕获，裂解和执行基因识别，所有这些都在同一设备上。图1显示了我们小组正在开发的整体集成生物芯片设备。 这些细胞和微生物的准确鉴定需要裂解细胞以提取用于分子鉴定的DNA和蛋白质。使用低功率且集成到微芯片格式上的电装置的DNA提取对于实现完全集成的生物分析装置可以是非常有用的。本项目的目标是设计和开发一种在手持设备内从病毒中提取遗传物质的方法，该设备功耗低，使用简单且紧凑。 我们将以牛痘病毒的西部储备株作为主要目标的模型病毒。作为一个延伸目标，我们将用人类冠状病毒进行实验，