Collaborative Research: Experimental and numerical studies of droplet formation and cell encapsulation in micro-channels for high-throughput electrical measurements
合作研究:微通道中液滴形成和细胞封装的实验和数值研究,用于高通量电测量
基本信息
- 批准号:1202325
- 负责人:
- 金额:$ 10.04万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2012
- 资助国家:美国
- 起止时间:2012-04-15 至 2016-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The objective of the research is to investigate and promote the use of pico-liter scale droplets for encapsulation and monitoring of bacterial growth, mammalian cell growth, and PCR reactions. The approach is to ascertain both experimentally and numerically, the device design, mechanisms of encapsulation, and biological reactions inside the droplets. The research is expected to result in increased understanding of the fundamental mechanisms as well as realization of practical devices for point-of-care diagnostics and screening applications.The intellectual merits of the proposed work include the development of novel numerical techniques for simulation of the cell encapsulation process, obtaining fundamental insight into the process of cell encapsulation in the droplets of cell media or buffer, examining the effect of ionic liquid medium on the cells, a systematic experiment and simulation approach to design the devices for optical capture, and whether single cell reactions can be interrogated inside these droplets.The broader impact of the proposed work is that a further understanding of the mechanism of cell encapsulation process in micro-channels will directly guide the use of droplets as reaction vessels for cells and molecules in biochemical applications. Thus, the proposed study can serves as generic platform that allows the biological activities to be monitored in real time using electrical means resulting in compact devices. The devices would be used to demonstrate fundamentals of microfluidics and bioassays to high school students in science fairs at OSU and as part of a microfluidic module in a 2 week long summer school at UIUC.
该研究的目的是调查和促进使用皮升规模的液滴封装和监测细菌生长,哺乳动物细胞生长和PCR反应。该方法是确定实验和数值,设备设计,封装机制,和液滴内的生物反应。该研究有望加深对基本机制的理解,并实现用于即时诊断和筛查应用的实用设备。拟议工作的智力价值包括开发用于模拟细胞包封过程的新型数值技术,获得对细胞包封在细胞培养基或缓冲液液滴中的过程的基本见解,考察了离子液体介质对细胞的影响,系统的实验和模拟方法来设计用于光学捕获的装置,以及是否可以在这些液滴内询问单细胞反应。所提出的工作的更广泛的影响是,进一步了解微滴中细胞包封过程的机制,通道将直接引导液滴在生物化学应用中用作细胞和分子的反应容器。因此,所提出的研究可以作为通用平台,其允许使用电气手段在真实的时间内监测生物活性,从而产生紧凑的装置。这些设备将用于在俄勒冈州立大学的科学博览会上向高中生展示微流体和生物测定的基础知识,并作为UIUC为期2周的暑期学校的微流体模块的一部分。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Rashid Bashir其他文献
Nanopore sensors for nucleic acid analysis
用于核酸分析的纳米孔传感器
- DOI:
10.1038/nnano.2011.129 - 发表时间:
2011-09-18 - 期刊:
- 影响因子:34.900
- 作者:
Bala Murali Venkatesan;Rashid Bashir - 通讯作者:
Rashid Bashir
Detection of Methylation on dsDNA at Single-Molecule Level using Solid-State Nanopores
- DOI:
10.1016/j.bpj.2017.11.1205 - 发表时间:
2018-02-02 - 期刊:
- 影响因子:
- 作者:
Julian Bello;younghoon Kim;Shouvik Banerjee;Kirby Smithe;David Estrada;SuA Myong;Ann Nardulli;Eric Pop;Rashid Bashir;Jiwook Shim - 通讯作者:
Jiwook Shim
Detection of Methylated DNA by Modified GP10 Nanopore
- DOI:
10.1016/j.bpj.2011.11.1117 - 发表时间:
2012-01-31 - 期刊:
- 影响因子:
- 作者:
Elizabeth Wurtzler;Murali Venkatesan;Rashid Bashir;David Wendell - 通讯作者:
David Wendell
Leucyl-tRNA synthetase contributes to muscle weakness through mTORC1 activation and autophagy suppression in a mouse model of Duchenne Muscular Dystrophy.
在杜氏肌营养不良症小鼠模型中,亮氨酰-tRNA 合成酶通过 mTORC1 激活和自噬抑制导致肌肉无力。
- DOI:
- 发表时间:
2024 - 期刊:
- 影响因子:6
- 作者:
Jae;Kate Karaman;Adriana Reyes;Soohyun Lee;Yongdeok Kim;Rashid Bashir;Jie Chen - 通讯作者:
Jie Chen
Spatial mapping of cancer tissues by OMICS technologies
通过组学技术对癌组织进行空间映射
- DOI:
10.1016/j.bbcan.2021.188663 - 发表时间:
2022-01-01 - 期刊:
- 影响因子:8.300
- 作者:
Rashid Ahmed;Robin Augustine;Enrique Valera;Anurup Ganguli;Nasrin Mesaeli;Irfan S. Ahmad;Rashid Bashir;Anwarul Hasan - 通讯作者:
Anwarul Hasan
Rashid Bashir的其他文献
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{{ truncateString('Rashid Bashir', 18)}}的其他基金
Collaborative Research: Track 4: Developing Equity-Minded Engineering Practitioners (DEEP)
合作研究:轨道 4:培养具有公平意识的工程从业者 (DEEP)
- 批准号:
2308531 - 财政年份:2023
- 资助金额:
$ 10.04万 - 项目类别:
Continuing Grant
RAPID:COVID-19: RT-LAMP-based electrical detection of SARS-CoV-2
RAPID:COVID-19:基于 RT-LAMP 的 SARS-CoV-2 电检测
- 批准号:
2028431 - 财政年份:2020
- 资助金额:
$ 10.04万 - 项目类别:
Standard Grant
NSF IGERT: Training the Next Generation of Researchers in Cellular & Molecular Mechanics and Bionanotechnology
NSF IGERT:培训下一代细胞研究人员
- 批准号:
0965918 - 财政年份:2010
- 资助金额:
$ 10.04万 - 项目类别:
Continuing Grant
An Integrated Lab-on-a-Transistor for Biological Detection
用于生物检测的集成晶体管实验室
- 批准号:
1028549 - 财政年份:2010
- 资助金额:
$ 10.04万 - 项目类别:
Standard Grant
Integrated Nanowire Array Sensors for Biomolecular Detection
用于生物分子检测的集成纳米线阵列传感器
- 批准号:
0554990 - 财政年份:2006
- 资助金额:
$ 10.04万 - 项目类别:
Standard Grant
NER: Nano-Scale Probe Array for Extraction of Genetic Material from Viruses
NER:用于从病毒中提取遗传物质的纳米级探针阵列
- 批准号:
0404107 - 财政年份:2004
- 资助金额:
$ 10.04万 - 项目类别:
Standard Grant
Career: Silicon-Based Nano Structures and Bio-Sensors for the Nano-BioTechnology Era
职业:纳米生物技术时代的硅基纳米结构和生物传感器
- 批准号:
9984199 - 财政年份:2000
- 资助金额:
$ 10.04万 - 项目类别:
Standard Grant
NANOSCALE: Hybridization Based Assembly of Silicon Electronic Devices
纳米尺度:基于杂交的硅电子器件组装
- 批准号:
9986569 - 财政年份:2000
- 资助金额:
$ 10.04万 - 项目类别:
Standard Grant
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- 批准号:10774081
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- 资助金额:45.0 万元
- 项目类别:面上项目
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