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DISSERTATION RESEARCH: Virus Roles in Wild Cucurbita pepo and the Risks of Genetically Engineered Virus Resistance

论文研究：野生南瓜中的病毒作用以及基因工程病毒抗性的风险

基本信息

批准号：
0407610
负责人：
Alison Power
金额：
$ 1.21万
依托单位：
Cornell University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2004
资助国家：
美国
起止时间：
2004-06-01 至 2005-11-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0407610&HistoricalAwards=false
关键词：
DISSERTATION RESEARCH Virus Roles Wild

项目摘要

Although plant pathogens can significantly affect wild plants populations, the ecology of viruses in wild plant populations is rarely studied. Two ecological risks of genetically engineered (GE), virus-resistant crops (into which genes for virus-resistance have been inserted) prompt a need to understand the interactions of viruses within plants and the impact of viruses on wild plant populations. (1) If GE, virus-resistant crops interbreed with nearby, closely related wild plant populations, then GE virus resistance genes could enter wild populations of plants. If the targeted virus typically suppresses wild plant reproduction or survival, then virus- resistance may increase wild plant fitness and potentially increase the wild plant's invasiveness in natural and agricultural habitats.(2) If several viruses compete with each other to successfully infect a plant (crop or wild), then the plant's resistance to one virus could result in increased infection by formerly less competitive viruses. This project will examine a GE, virus-resistant squash (Cucurbita pepo) and a wild squash to examine the affect of viral infection in wild plant populations and to assess the associated risks of GE virus-resistance. In research to date, the PIs have confirmed with controlled experiments that GE virus-resistance increases wild squash fitness when virus infection is prevalent. In 2004, the PIs will determine the prevalence of virus infection in wild squash populations to predict the affect of GE virus-resistance on non-experimental, wild squash fitness. Prior research has detected competition between squash viruses. In 2004 and 2005, the PIs will conduct a series of greenhouse experiments to study this interaction more rigorously. This research will provide valuable information about virus ecology and assess potential ecological impacts of GE virus-resistance in crops.

虽然植物病原体可以显著影响野生植物种群，但野生植物种群中的病毒生态学研究很少。转基因抗病毒作物（其中插入了抗病毒基因）的两个生态风险促使人们需要了解病毒在植物中的相互作用以及病毒对野生植物种群的影响。 (1)如果转基因抗病毒作物与附近的、密切相关的野生植物种群杂交，那么转基因病毒抗性基因就可能进入野生植物种群。如果靶向病毒通常抑制野生植物繁殖或存活，则病毒抗性可增加野生植物适应性并潜在地增加野生植物在自然和农业栖息地中的入侵性。(2)如果几种病毒相互竞争成功感染一种植物（作物或野生），那么植物对一种病毒的抗性可能导致以前竞争力较弱的病毒感染增加。该项目将研究转基因、抗病毒南瓜（西葫芦）和野生南瓜，以研究病毒感染对野生植物种群的影响，并评估转基因抗病毒的相关风险。在迄今为止的研究中，PI已经通过对照实验证实，当病毒感染普遍时，GE病毒抗性增加了野生南瓜的适合度。 2004年，PI将确定野生南瓜种群中病毒感染的流行程度，以预测转基因病毒抗性对非实验野生南瓜适合度的影响。先前的研究已经发现了南瓜病毒之间的竞争。在2004年和2005年，PI将进行一系列温室实验，以更严格地研究这种相互作用。这项研究将提供有关病毒生态学的有价值的信息，并评估作物抗转基因病毒的潜在生态影响。