Microbial Genome Sequencing:Wheat Stem Rust Fungus Genome Sequencing Project

微生物基因组测序：小麦茎锈菌基因组测序计划

• 批准号: 0412264
• 负责人: Les Szabo
• 金额: --
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2010-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0412264&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Wheat; Stem

Rust fungi (Uredinales) are obligate plant parasites with a wide range of plant hosts that include ferns, gymnosperms and angiosperms. Puccinia graminis f.sp. tritici, the causal agent of stem rust of wheat, has been the most devastating fungal pathogen of wheat. Epidemics of the early 1950's destroyed more than 400 million bushels of wheat in North America. The results of this project will enable the use of genomic and other approaches to elucidate the mechanisms of pathogenicity and the nature of the biotrophic life-style of rust fungi. The specific objectives of this research are: (1) to produce an assembled genome sequence of one isolate of P. graminis f.sp. tritici with at least 8X coverage; (2) to develop a physical map of P. graminis via DNA fingerprinting fosmid clones; (3) to develop 40,000 ESTs from full length cDNA libraries representing four different stages of fungal development; (4) to integrate EST and, genetic and physical mapping data with the assembled sequence; (5) to annotate the genomic sequence and make these data publicly available through user friendly web-based tools and interfaces; and (6) to train undergraduate students and Postdoctoral Research Fellows in bioinformatics and fungal genomics.

锈菌目 (Uredinales) 是专性植物寄生虫，其寄主范围广泛，包括蕨类植物、裸子植物和被子植物。 禾本科柄锈菌小麦是小麦茎锈病的致病菌，是小麦最具破坏性的真菌病原体。 1950 年代初期的流行病摧毁了北美 4 亿多蒲式耳的小麦。 该项目的结果将能够利用基因组和其他方法来阐明锈菌的致病机制和生物营养生活方式的性质。 本研究的具体目标是：(1) 产生禾谷赤霉专化型(P. graminis f.sp) 的一个分离株的组装基因组序列。小麦覆盖率至少为 8 倍； (2) 通过 DNA 指纹 fosmid 克隆绘制 P. graminis 的物理图谱； (3) 从代表真菌发育的四个不同阶段的全长cDNA文库中开发40,000个EST； (4)将EST、遗传和物理图谱数据与组装的序列整合； (5) 注释基因组序列并通过用户友好的基于网络的工具和界面公开这些数据； （6）培养生物信息学和真菌基因组学方面的本科生和博士后研究员。