Sequence and Annotation of the Euchromatin of Tomato.

番茄基因组含有约9.5亿个碱基对的DNA，其中75%以上是密集排列的，基本上不存在基因。大多数基因是在位于每个染色体臂远端的富含基因的DNA的长长的连续延伸中发现的。在这个项目中，我们将为一个国际联盟对所有12条番茄染色体的富含基因的区域进行测序奠定基础，该联盟是茄科基因组学计划或“SOL”(http://www.sgn.cornell.edu/solanaceae-project/).的一部分该项目将从剪切的番茄DNA文库中生成序列，以更深入地了解番茄基因组的结构，并通过对40万条细菌人工染色体(BAC)的序列结束来生成序列连接符数据库。此外，从美国5条染色体中精心挑选的20个BAC将被完全测序。为了进一步确定番茄中的基因丰富区域，将对大约50个选定的BAC进行荧光原位杂交(FISH)分析。茄科基因组网络(SGN，http://www.sgn.cornell.edu/)，)是一个专门研究茄科物种基因组学的关系数据库和网站，该网络将扩大，以容纳和纳入该项目产生的所有测序、注释和绘图信息。

The genome of tomato (Solanum lycopersicon) contains approximately 950 million base pairs of DNA, more than 75% of which is densely packed and largely devoid of genes. The majority of genes are found in long contiguous stretches of gene-rich DNA located on the distal portions of each chromosome arm. In this project, we will lay the foundation for the sequencing of the gene-rich regions of all 12 tomato chromosomes by an international consortium as part of the Solanaceae Genomics Project or "SOL" (http://www.sgn.cornell.edu/solanaceae-project/). The project will generate sequences from a sheared tomato DNA library to gain more insight into the structure of the tomato genome, and sequence 400,000 Bacterial Artificial Chromosome (BAC) ends to generate a sequence connector database. In addition, 20 carefully chosen BACs from the 5 US chromosomes will be fully sequenced. Fluorescence in-situ hybridization (FISH) analysis will be performed with about 50 selected BACs to further define the gene-rich regions in tomato. The Solanaceae Genome Network (SGN, http://www.sgn.cornell.edu/), a relational database and website devoted to the genomics of solanaceous species, will be expanded to accommodate and incorporate all of the sequencing, annotation and mapping information generated from this project.