Sequence and Annotation of the Euchromatin of Tomato.

番茄（Solanum lycopersicon）的基因组包含大约9.5亿碱基对的DNA，其中75%以上是密集排列的，大部分缺乏基因。大多数基因位于每个染色体臂的远端部分的富含基因的DNA的长连续延伸中。在这个项目中，我们将为一个国际联盟对所有12个番茄染色体的基因丰富区域进行测序奠定基础，该联盟是Solanaceae基因组学项目或“SOL”（http://www.sgn.cornell.edu/solanaceae-project/）的一部分。该项目将从一个剪切的番茄DNA文库中生成序列，以更深入地了解番茄基因组的结构，并对40万个细菌人工染色体（BAC）末端进行测序，以生成序列连接器数据库。此外，从5条美国染色体中精心挑选的20个bac将被完全测序。选取50个左右的bac进行荧光原位杂交（FISH）分析，进一步确定番茄中基因丰富的区域。茄科基因组网络（SGN, http://www.sgn.cornell.edu/）是一个致力于茄科物种基因组学的关系数据库和网站，将被扩展以容纳并纳入该项目产生的所有测序、注释和制图信息。

The genome of tomato (Solanum lycopersicon) contains approximately 950 million base pairs of DNA, more than 75% of which is densely packed and largely devoid of genes. The majority of genes are found in long contiguous stretches of gene-rich DNA located on the distal portions of each chromosome arm. In this project, we will lay the foundation for the sequencing of the gene-rich regions of all 12 tomato chromosomes by an international consortium as part of the Solanaceae Genomics Project or "SOL" (http://www.sgn.cornell.edu/solanaceae-project/). The project will generate sequences from a sheared tomato DNA library to gain more insight into the structure of the tomato genome, and sequence 400,000 Bacterial Artificial Chromosome (BAC) ends to generate a sequence connector database. In addition, 20 carefully chosen BACs from the 5 US chromosomes will be fully sequenced. Fluorescence in-situ hybridization (FISH) analysis will be performed with about 50 selected BACs to further define the gene-rich regions in tomato. The Solanaceae Genome Network (SGN, http://www.sgn.cornell.edu/), a relational database and website devoted to the genomics of solanaceous species, will be expanded to accommodate and incorporate all of the sequencing, annotation and mapping information generated from this project.