CAREER: Using Systems Biology to Study MAP Kinase Cascades in Arabidopsis

职业：利用系统生物学研究拟南芥中的 MAP 激酶级联

• 批准号: 0447750
• 负责人: Patrick Krysan
• 金额: $ 70万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-15 至 2011-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0447750&HistoricalAwards=false
• 关键词: CAREER; Using; Systems; Biology; Study

The research team will use a systems biology approach to understand the process of MAP kinase (MAPK) signal transduction in Arabidopsis. Whole-genome sequencing has indicated that there are 20 genes that encode MAPK proteins in Arabidopsis. Functional redundancy and cross-talk between these proteins make it difficult to determine which signaling pathways are controlled by a given protein. In order to overcome this complexity, the research team will use a novel high-throughput genotyping pipeline to create single plants that are heterozygous for mutations in all twenty MAPK genes. The progeny of these 20-tuple heterozygotes should segregate all of the possible combinations of MAPK mutations. By genotyping selected sets of progeny, it should be possible to determine which mutant combinations are causing particular phenotypes. The research team will not search through the population to try and isolate examples of all 1,140 different triple-mutants. Instead they will let the biology of the plants indicate which mutant combinations are informative. The outcome of this work will be an improved understanding of the many signal transduction pathways controlled by MAPK cascades in Arabidopsis. Educational materials will also be developed that highlight the concepts of systems biology and high-throughput technology. Dr. Krysan will incorporate the high-throughput genotyping system into his Plant Functional Genomics course in order to give students hands-on experience with these methods. At the high school level, a local teacher will collaborate with the research team to develop practical exercises suitable for use in a high school setting that capture the essence of high-throughput genotyping. These materials will be disseminated to other high school teachers through a summer workshop that will be offered.

研究团队将使用系统生物学方法来了解拟南芥中MAP激酶（MAPK）信号转导的过程。 全基因组测序表明，有20个基因在拟南芥中编码MAPK蛋白。 这些蛋白质之间的功能冗余和串扰使得难以确定哪些信号通路由给定蛋白控制。 为了克服这种复杂性，研究团队将使用一种新型的高通量基因分型管道来创建单个植物，这些植物是所有20个MAPK基因突变的杂合子。这些20元组杂合子的后代应隔离MAPK突变的所有可能组合。通过为选定的后代集基因分型，应该可以确定哪些突变组合引起了特定的表型。研究小组将不会在人群中进行搜索，以尝试隔离所有1,140种不同三重突击剂的例子。相反，他们将让植物的生物学指出哪些突变组合有用。 这项工作的结果将是对MAPK Cascades在拟南芥中控制的许多信号转导途径的了解。 还将开发教育材料，以突出系统生物学和高通量技术的概念。 Krysan博士将将高通量基因分型系统纳入其植物功能基因组学课程中，以便为学生提供这些方法的动手体验。 在高中一级，当地老师将与研究团队合作，开发适合在高中毕业环境中使用的实践练习，以捕捉高通量基因分型的本质。这些材料将通过将提供的夏季研讨会传播给其他高中老师。