CAREER: Using Systems Biology to Study MAP Kinase Cascades in Arabidopsis

职业：利用系统生物学研究拟南芥中的 MAP 激酶级联

• 批准号: 0447750
• 负责人: Patrick Krysan
• 金额: $ 70万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-15 至 2011-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0447750&HistoricalAwards=false
• 关键词: CAREER; Using; Systems; Biology; Study

The research team will use a systems biology approach to understand the process of MAP kinase (MAPK) signal transduction in Arabidopsis. Whole-genome sequencing has indicated that there are 20 genes that encode MAPK proteins in Arabidopsis. Functional redundancy and cross-talk between these proteins make it difficult to determine which signaling pathways are controlled by a given protein. In order to overcome this complexity, the research team will use a novel high-throughput genotyping pipeline to create single plants that are heterozygous for mutations in all twenty MAPK genes. The progeny of these 20-tuple heterozygotes should segregate all of the possible combinations of MAPK mutations. By genotyping selected sets of progeny, it should be possible to determine which mutant combinations are causing particular phenotypes. The research team will not search through the population to try and isolate examples of all 1,140 different triple-mutants. Instead they will let the biology of the plants indicate which mutant combinations are informative. The outcome of this work will be an improved understanding of the many signal transduction pathways controlled by MAPK cascades in Arabidopsis. Educational materials will also be developed that highlight the concepts of systems biology and high-throughput technology. Dr. Krysan will incorporate the high-throughput genotyping system into his Plant Functional Genomics course in order to give students hands-on experience with these methods. At the high school level, a local teacher will collaborate with the research team to develop practical exercises suitable for use in a high school setting that capture the essence of high-throughput genotyping. These materials will be disseminated to other high school teachers through a summer workshop that will be offered.

该研究小组将使用系统生物学方法来了解拟南芥中MAP激酶（MAPK）信号转导的过程。 全基因组测序表明，拟南芥中有20个编码MAPK蛋白的基因。 这些蛋白质之间的功能冗余和串扰使得难以确定哪些信号通路由给定蛋白质控制。 为了克服这种复杂性，研究小组将使用一种新的高通量基因分型管道来创建所有20个MAPK基因突变杂合的单株。这些20-tuple杂合子的后代应该分离所有可能的MAPK突变组合。通过对选定的后代进行基因分型，应该可以确定哪些突变体组合引起特定的表型。研究小组不会在整个种群中进行搜索，试图分离出所有1,140种不同的三重突变体。相反，他们将让植物的生物学表明哪些突变组合是有信息的。 这项工作的结果将是一个更好的了解许多信号转导途径控制的MAPK级联在拟南芥。 还将编制教育材料，突出系统生物学和高通量技术的概念。 Krysan博士将把高通量基因分型系统纳入他的植物功能基因组学课程，以便让学生亲身体验这些方法。 在高中阶段，一名当地教师将与研究团队合作，开发适合在高中环境中使用的实用练习，以捕捉高通量基因分型的本质。这些材料将通过将提供的暑期讲习班分发给其他高中教师。