BTT EAGER: A system for the production of haploid inducer lines and cytoplasmic male sterile doubled haploids for efficient hybrid production

BTT EAGER:用于生产单倍体诱导系和细胞质雄性不育双单倍体的系统,用于高效杂交生产

基本信息

  • 批准号:
    1844304
  • 负责人:
  • 金额:
    $ 30万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-03-01 至 2023-02-28
  • 项目状态:
    已结题

项目摘要

Hybrid cultivars are a cornerstone of modern crop production. Seed companies spend many years developing elite, inbred parental lines that are used to produce the hybrid seed that farmers plant each season. A major bottleneck in the plant breeding cycle is caused by the fact that it takes many years to produce these inbred parental lines. The aim of this project is to develop a shortcut that allows inbred parental lines to be produced in a single year. To accomplish this goal, the investigators plan to use gene editing techniques to generate novel onion plants that greatly accelerate the process of inbred line development. The strategy deployed in this work should also be broadly applicable to other crops, making it of general use to the plant breeding community. This work will be carried out in collaboration with Dr. Stephen Jackson at the University of Warwick.The investigators will use CRISPR/Cas9 methods to introduce genetic variation into the CENH3 gene of onion in order to produce haploid-inducer lines. A haploid-inducer line is a plant from which a significant fraction of the seed produced by cross-pollination is haploid. Upon chemically-induced chromosome doubling, these haploid progeny become "instant inbred" lines that can be immediately used for hybrid seed production. The research team at the University of Wisconsin will use T-DNA transformation and particle bombardment methods to deliver the gene editing reagents into onion somatic embryos, while the group at the University of Warwick will use tobacco rattle virus (TRV) expression vectors. Deploying a variety of gene-editing strategies in parallel should allow the investigators to quickly determine which approach is the most effective for efficiently producing modified CENH3 alleles. In order for doubled haploid technology to be commercially useful for hybrid seed production, it is essential that the strategies for producing doubled haploids are compatible with cytoplasmic male sterile breeding systems. For this reason, this project will integrate the production of haploid inducer lines within a cytoplasmic male sterile breeding framework in order to maximize the practical benefit and applicability to breeders.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
杂交品种是现代作物生产的基石。种子公司花了很多年的时间来开发优良的近交亲本系,用于生产农民每个季节种植的杂交种子。植物育种周期中的一个主要瓶颈是由需要多年来产生这些近交亲本系的事实引起的。 该项目的目的是开发一种捷径,使近交亲本系在一年内产生。 为了实现这一目标,研究人员计划使用基因编辑技术来产生新的洋葱植物,从而大大加快自交系发育的过程。 在这项工作中部署的战略也应该广泛适用于其他作物,使其普遍使用的植物育种界。这项工作将与沃里克大学的斯蒂芬杰克逊博士合作进行。研究人员将使用CRISPR/Cas9方法将遗传变异引入洋葱的CENH 3基因,以产生单倍体诱导系。单倍体诱导系是通过异花授粉产生的种子的显著部分是单倍体的植物。 在化学诱导的染色体加倍后,这些单倍体后代成为可以立即用于杂交种子生产的“即时近交”系。 威斯康星州大学的研究小组将使用T-DNA转化和粒子轰击方法将基因编辑试剂输送到洋葱体细胞胚中,而沃里克大学的研究小组将使用烟草拨浪鼓病毒(TRV)表达载体。 并行部署各种基因编辑策略应该使研究人员能够快速确定哪种方法对于有效地产生修饰的CENH 3等位基因最有效。 为了使双单倍体技术在商业上可用于杂交种子生产,生产双单倍体的策略必须与细胞质雄性不育育种系统相容。因此,该项目将在细胞质雄性不育育种框架内整合单倍体诱导系的生产,以最大限度地提高育种者的实际效益和适用性。该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

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Patrick Krysan其他文献

Patrick Krysan的其他文献

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{{ truncateString('Patrick Krysan', 18)}}的其他基金

Collaborative Research: Assessing the connections between genetic interactions, environments, and phenotypes in Arabidopsis thaliana
合作研究:评估拟南芥遗传相互作用、环境和表型之间的联系
  • 批准号:
    2210432
  • 财政年份:
    2022
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
Collaborative Research: Fitness effects of loss-of-function mutations in duplicate genes
合作研究:重复基因功能丧失突变的适应性影响
  • 批准号:
    1655630
  • 财政年份:
    2017
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
EAGER: Engineering Chromosomal Deletions in Arabidopsis Plants using Unequal Crossovers
EAGER:使用不等交叉在拟南芥植物中进行染色体缺失工程
  • 批准号:
    1407063
  • 财政年份:
    2014
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
EAGER: Developing a Fluorescent Biosensor to Enable Real-Time Analysis of MAP Kinase Signaling Pathways in Living Arabidopsis Plants
EAGER:开发荧光生物传感器以实时分析活体拟南芥植物中的 MAP 激酶信号通路
  • 批准号:
    1137950
  • 财政年份:
    2012
  • 资助金额:
    $ 30万
  • 项目类别:
    Continuing Grant
Arabidopsis 2010: A Community Resource for Generating Targeted Deletions Throughout the Arabidopsis Genome
拟南芥 2010:用于在整个拟南芥基因组中生成靶向删除的社区资源
  • 批准号:
    0514171
  • 财政年份:
    2005
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
CAREER: Using Systems Biology to Study MAP Kinase Cascades in Arabidopsis
职业:利用系统生物学研究拟南芥中的 MAP 激酶级联
  • 批准号:
    0447750
  • 财政年份:
    2005
  • 资助金额:
    $ 30万
  • 项目类别:
    Continuing Grant
Postdoctoral Research Fellowship in Plant Biology
植物生物学博士后研究奖学金
  • 批准号:
    9404035
  • 财政年份:
    1994
  • 资助金额:
    $ 30万
  • 项目类别:
    Fellowship Award

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EAGER: Generalizing Monin-Obukhov Similarity Theory (MOST)-based Surface Layer Parameterizations for Turbulence Resolving Earth System Models (ESMs)
EAGER:将基于 Monin-Obukhov 相似理论 (MOST) 的表面层参数化推广到湍流解析地球系统模型 (ESM)
  • 批准号:
    2414424
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
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    Standard Grant
Collaborative Research: EAGER--Evaluation of Optimal Mesonetwork Design for Monitoring and Predicting North American Monsoon (NAM) Convection Using Observing System Simulation
合作研究:EAGER——利用观测系统模拟监测和预测北美季风(NAM)对流的最佳中观网络设计评估
  • 批准号:
    2308410
  • 财政年份:
    2023
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
EAGER: Privacy Preserving Synthetic Graph Generation for System Provenance
EAGER:用于系统起源的隐私保护合成图生成
  • 批准号:
    2331424
  • 财政年份:
    2023
  • 资助金额:
    $ 30万
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    Continuing Grant
EAGER-QAC-QSA: Quantum Algorithms for Correlated Electron-Phonon System
EAGER-QAC-QSA:相关电子声子系统的量子算法
  • 批准号:
    2337930
  • 财政年份:
    2023
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
EAGER: Toward a Decentralized Cross-administrator Zone Management System: Policy and Technology
EAGER:走向去中心化的跨管理员区域管理系统:政策和技术
  • 批准号:
    2331936
  • 财政年份:
    2023
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
Collaborative Research: EAGER--Evaluation of Optimal Mesonetwork Design for Monitoring and Predicting North American Monsoon (NAM) Convection Using Observing System Simulation
合作研究:EAGER——利用观测系统模拟监测和预测北美季风(NAM)对流的最佳中观网络设计评估
  • 批准号:
    2308409
  • 财政年份:
    2023
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    $ 30万
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    Standard Grant
EAGER: A hybrid dialogue system architecture for symbolic control of deep learning networks
EAGER:用于深度学习网络符号控制的混合对话系统架构
  • 批准号:
    2232307
  • 财政年份:
    2022
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
EAGER: A New Multiphoton Luminescence Imaging Approach to Interrogate the Fate of Metallic Nanoparticles in a Living System
EAGER:一种新的多光子发光成像方法来探究金属纳米粒子在生命系统中的命运
  • 批准号:
    2223834
  • 财政年份:
    2022
  • 资助金额:
    $ 30万
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EAGER: Towards a Homeostatic Nanobio-Hybrid Mechanical System
EAGER:迈向稳态纳米生物混合机械系统
  • 批准号:
    2230116
  • 财政年份:
    2022
  • 资助金额:
    $ 30万
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EAGER/Collaborative Research: Enable Elastic Capacity for Transportation Infrastructure through a Transmodal Modular Autonomous Vehicle System
EAGER/协作研究:通过跨模式模块化自动驾驶车辆系统实现交通基础设施的弹性能力
  • 批准号:
    2313835
  • 财政年份:
    2022
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
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