CAREER: Developing Asymmetric-Polarization AC Electroosmosis for Lab-on-a-chip

职业：为芯片实验室开发非对称偏振交流电渗

• 批准号: 0448896
• 负责人: Jie Wu
• 金额: --
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0448896&HistoricalAwards=false
• 关键词: CAREER; Developing; Asymmetric; Polarization; AC

The research objective is to develop and validate an electrokinetic lab-on-chip forrobust and rapid detection of low concentration bioparticle (eg. virus, bacteria, cells) inwater, food and clinical samples. The research tackles the long-standing bottleneckissues of sensitivity and selectivity for such detection. The approach is: 1) to differentiatebioparticles by their response to electrokinetic transport based on various charge/massratio, 2) to rapidly enrich local particle concentration by expediting bioparticles' diffusionto sensors with convection, and 3) to quantify particle concentration by impedimetricmeasurement while incorporating electrokinetic signal amplification. The abovefunctions are to be realized by asymmetric-polarization AC electro-osmosis, which isnewly discovered and at the forefront of electrokinetic microfluidic research. Such lab-on-a-chip operates at small voltage with low power consumption, and its all electricalprocesses facilitate portability, parallelism and automation for on-site bioparticledetection.Bioparticle detection is of great significance because bioparticles often serve ascarrier/indicator of pathogens and/or toxins. As the world becomes increasinglyconcerned with toxin/pathogenic contamination in food, water and environment frominfectious diseases and bioterrorism, and because the threat of pathogen/toxins tosocieties could be substantially mitigated with early detection, the demand for rapidbioparticle detection is expanding for both civilian and military applications. The PIproposes a novel electrokinetic lab-on-a-chip to detect bioparticles at low concentrations.The research will not only lead to a practical detection chip but also afford students aunique opportunity to explore the state-of-art of interdisciplinary research inmicrosystem, bio/chemi/electronics and microfluidics.

本研究的目的是开发和验证一种用于低浓度生物颗粒（如大肠杆菌）检测的电动实验室芯片。病毒、细菌、细胞）。这项研究解决了长期存在的敏感性和选择性问题。方法是：1）根据生物颗粒对基于不同电荷/质量比的电动传输的响应来区分生物颗粒，2）通过加速生物颗粒向具有对流的传感器的扩散来快速富集局部颗粒浓度，以及3）通过阻抗测量同时结合电动信号放大来量化颗粒浓度。上述功能是通过非对称极化交流电渗来实现的，这是一种新发现的，处于电动微流体研究的前沿。这种芯片实验室的工作电压低、功耗低，其全电过程便于现场生物颗粒检测的便携性、并行性和自动化，生物颗粒检测具有重要意义，因为生物颗粒往往是病原体和/或毒素的载体/指示物。随着世界越来越关注传染病和生物恐怖主义对食物、水和环境的毒素/病原体污染，并且由于病原体/毒素对社会的威胁可以通过早期检测而大大减轻，因此对快速生物颗粒检测的需求在民用和军用方面都在扩大。本项目提出了一种新型的电动实验室芯片，用于检测低浓度的生物颗粒。该研究不仅将导致实用的检测芯片，而且还将为学生提供一个独特的机会，探索微系统，生物/化学/电子学和微流体学的跨学科研究。