Metal Phosphonate Surfaces for Bioaray Applications

佛罗里达大学的Daniel Talham教授在分析和表面化学项目的支持下，对生物阵列应用中磷酸锆表面的探针固定化进行了表征和优化。他们的想法是开发DNA序列传感器，最终用于蛋白质。关于表面覆盖、探针密度、特异性与非特异性结合、靶捕获和杂交效率、不同探针条件下的动力学、DNA双链的固定化和蛋白质的固定化等问题正在进行研究。方法包括x射线光发射光谱、石英晶体微天平、原子力显微镜和荧光成像。PI与法国南特大学和一家法国公司合作。这个项目的结果将增加我们对生物阵列技术的理解，这是医学诊断和检测应用的有用工具。

Professor Daniel Talham of the University of Florida is supported by the Analytical and Surface Chemistry Program to characterize and to optimize probe immobilization at zirconium phosphonate surfaces for bioarray applications. The idea is to develop sensors for DNA sequences and eventually proteins. Issues about surface coverage, probe density, specific vs non-specific binding, target capture and hybridication efficiency, kinetics for different probe conditions, immobilization of DNA duplexes, and immobilization of proteins are being examined. Approaches include X-ray photoemission spectroscopy, quartz crystal microbalance, atomic force microscopy and fluorescence imaging. The PI collaborates with the Universite de Nantes in France and a French company.The results of this project will increase our understanding of bioarray technologies, which are useful tools for medical diagnostic and detection applications.