Metal Phosphonate Surfaces for Bioaray Applications

用于生物阵列应用的金属磷酸盐表面

• 批准号: 0514437
• 负责人: Daniel Talham
• 金额: $ 37万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-15 至 2009-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0514437&HistoricalAwards=false
• 关键词: Metal; Phosphonate; Surfaces; Bioaray; Applications

Professor Daniel Talham of the University of Florida is supported by the Analytical and Surface Chemistry Program to characterize and to optimize probe immobilization at zirconium phosphonate surfaces for bioarray applications. The idea is to develop sensors for DNA sequences and eventually proteins. Issues about surface coverage, probe density, specific vs non-specific binding, target capture and hybridication efficiency, kinetics for different probe conditions, immobilization of DNA duplexes, and immobilization of proteins are being examined. Approaches include X-ray photoemission spectroscopy, quartz crystal microbalance, atomic force microscopy and fluorescence imaging. The PI collaborates with the Universite de Nantes in France and a French company.The results of this project will increase our understanding of bioarray technologies, which are useful tools for medical diagnostic and detection applications.

佛罗里达大学的丹尼尔塔尔汉姆教授得到了分析和表面化学项目的支持，以表征和优化生物阵列应用中膦酸锆表面的探针固定。 这个想法是开发DNA序列和最终蛋白质的传感器。 关于表面覆盖率，探针密度，特异性与非特异性结合，目标捕获和杂交效率，不同探针条件下的动力学，DNA双链体的固定化和蛋白质的固定化的问题正在研究中。 方法包括X射线光电子能谱、石英晶体微天平、原子力显微镜和荧光成像。 PI与法国南特大学和一家法国公司合作，该项目的结果将增加我们对生物阵列技术的理解，生物阵列技术是医学诊断和检测应用的有用工具。