Metal Phosphonate Interfaces for Phosphopeptide Enrichment

用于磷酸肽富集的金属磷酸盐界面

• 批准号: 0957155
• 负责人: Daniel Talham
• 金额: $ 39.25万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0957155&HistoricalAwards=false
• 关键词: Metal; Phosphonate; Interfaces; Phosphopeptide; Enrichment

In this project supported by the Chemical Measurement and Imaging program (with co-funding from the Office of International Science and Engineering), Professor Daniel Talham and coworkers at the University of Florida are exploring metal phosphate and metal phosphonate solids and thin films as a new class of phosphopeptide separation and enrichment media. The expected improvements in performance are based on a new mechanism for phosphopeptide affinity involving the ability of some metal phosphate solids to selectively form covalent linkages to divalent phosphate groups while excluding other common anions. The project will explore new particle systems for affinity enrichment and new surface modification approaches to extend metal phosphonate-based enrichment strategies to on-target techniques. The new mechanisms and materials will be evaluated using surface analytical methods to quantify the activity of the new materials toward different phosphopeptide structures. Finally, newly developed systems will be tested to prove their applicability to phosphoprotein mass spectrometry.These experiments seek to devise new means of isolating a wide range of biomaterials that are important in high-technology applications ranging from sensing and diagnostics to enabling fundamental studies of proteins - a fundamental building block of life. In addition, training related to biomaterials, interfaces, chemical measurement, and protein chemistry will provide graduate and undergraduate students with the skills and knowledge needed to be competitive in biotechnology related high-technology professions. The project also fosters collaboration with international partners at the Université de Nantes in France. This cooperation includes an extensive international educational component through the exchange of graduate student researchers. These exchanges are designed to facilitate thesis work and to also provide students with important alternative perspectives on research and approaches to scientific exploration.

在这个由化学测量和成像项目（由国际科学与工程办公室共同资助）支持的项目中，佛罗里达大学的Daniel Talham教授和他的同事正在探索金属磷酸盐和金属膦酸盐固体和薄膜作为一类新的磷酸肽分离和富集介质。预期的性能改进是基于一种新的磷酸肽亲和机制，涉及一些金属磷酸固体选择性地与二价磷酸基团形成共价键，同时排除其他常见阴离子的能力。该项目将探索用于亲和富集的新粒子系统和新的表面改性方法，以将基于金属膦酸盐的富集策略扩展到靶技术。新的机制和材料将使用表面分析方法进行评估，以量化新材料对不同磷酸肽结构的活性。最后，将对新开发的系统进行测试，以证明其对磷蛋白质谱的适用性。这些实验试图设计出新的方法来分离各种各样的生物材料，这些材料在从传感和诊断到蛋白质（生命的基本组成部分）的基础研究等高科技应用中都很重要。此外，与生物材料、界面、化学测量和蛋白质化学相关的培训将为研究生和本科生提供在生物技术相关的高科技行业中具有竞争力所需的技能和知识。该项目还促进了与法国南特大学的国际合作伙伴的合作。这一合作包括通过研究生研究人员的交流进行广泛的国际教育组成部分。这些交流旨在促进论文工作，并为学生提供重要的研究和科学探索方法的替代观点。