Metal Phosphonate Interfaces for Phosphopeptide Enrichment

在这个由化学测量和成像计划(由国际科学与工程办公室共同资助)支持的项目中，佛罗里达大学的Daniel Talham教授和他的同事们正在探索金属磷酸盐和金属磷酸盐固体和薄膜作为一种新型的磷肽分离和浓缩介质。预期的性能改进是基于一种新的磷肽亲和力机制，该机制涉及一些金属磷酸盐固体选择性地与二价磷酸基团形成共价键的能力，同时排除其他常见的阴离子。该项目将探索用于亲和浓缩的新粒子系统和新的表面修饰方法，以将基于金属膦的浓缩策略扩展到靶标技术。新的机理和材料将使用表面分析方法进行评估，以量化新材料对不同磷肽结构的活性。最后，新开发的系统将接受测试，以证明它们对磷蛋白质谱学的适用性。这些实验试图设计出分离各种生物材料的新方法，这些材料在从传感和诊断到实现对蛋白质的基础研究--生命的基本构件--的高科技应用中非常重要。此外，与生物材料、界面、化学测量和蛋白质化学相关的培训将为研究生和本科生提供在生物技术相关高科技职业中具有竞争力所需的技能和知识。该项目还促进了与法国南特大学国际合作伙伴的合作。这种合作包括通过交换研究生研究人员进行广泛的国际教育。这些交流旨在促进论文工作，并为学生提供关于研究和科学探索方法的重要替代观点。

In this project supported by the Chemical Measurement and Imaging program (with co-funding from the Office of International Science and Engineering), Professor Daniel Talham and coworkers at the University of Florida are exploring metal phosphate and metal phosphonate solids and thin films as a new class of phosphopeptide separation and enrichment media. The expected improvements in performance are based on a new mechanism for phosphopeptide affinity involving the ability of some metal phosphate solids to selectively form covalent linkages to divalent phosphate groups while excluding other common anions. The project will explore new particle systems for affinity enrichment and new surface modification approaches to extend metal phosphonate-based enrichment strategies to on-target techniques. The new mechanisms and materials will be evaluated using surface analytical methods to quantify the activity of the new materials toward different phosphopeptide structures. Finally, newly developed systems will be tested to prove their applicability to phosphoprotein mass spectrometry.These experiments seek to devise new means of isolating a wide range of biomaterials that are important in high-technology applications ranging from sensing and diagnostics to enabling fundamental studies of proteins - a fundamental building block of life. In addition, training related to biomaterials, interfaces, chemical measurement, and protein chemistry will provide graduate and undergraduate students with the skills and knowledge needed to be competitive in biotechnology related high-technology professions. The project also fosters collaboration with international partners at the Université de Nantes in France. This cooperation includes an extensive international educational component through the exchange of graduate student researchers. These exchanges are designed to facilitate thesis work and to also provide students with important alternative perspectives on research and approaches to scientific exploration.