Integrated Transduction, Actuation, and Control for Cell-Based Sensing [UOM_FY05_059]

基于细胞的传感的集成转导、驱动和控制 [UOM_FY05_059]

• 批准号: 0515873
• 负责人: Pamela Abshire
• 金额: $ 31.13万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-15 至 2015-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0515873&HistoricalAwards=false
• 关键词: Integrated; Transduction; Actuation; Control; Cell

ABSTRACTIIS-0515873Abshire, PamelaThe goal is to develop and demonstrate enabling technology for cell-basedsensing. Cell clinics are microenvironments that enable the capture and characterization of cells. Each "clinic" is a micro-electro-mechanical system fabricated on a CMOS chip. Biological systems have high specificity, sensitivity, and adaptability that can be part of a highly integrated sensor. The first goals are sample preparation, cell loading, and system miniaturization using the tools of feedback control, integrated circuits, and microfluidics. Results will leveraged into two ongoing efforts in olfactory sensing and low-false-positive pathogen detection. Three aspects of the system will be demonstrated. (1) Electroosmotic flow control will remove all optically visible (5 micron) particles from the sample. This will remove dirt, dust, and bacteria and leave behind odorants for presentation to the olfactory cell sensors. This system shall be capable of sufficiently high throughput to be used in real time. (2) Dielectrophoretic actuation for steering cells in three-dimensions will be used to position cells in the plane and to direct them into the cell clinic vials. (3)In order to develop field utility cell-based sensors, a vision system with the same dimensions as cell clinics for cell steering will be developed. The proposed technological advances will allow cell-based sensing to move toward actual implementation and use with real samples. Cell-based sensing has the potential for selectivity, sensitivity, and speed that far exceedtoday's chemical and biological sensors. Problems of olfactory sensing and pathogen detection are of immediate relevance to national security. This technology has clear applications in other diverse fields such as health care, pharmaceutical development, and environmental monitoring. The integrated transduction-actuation-control approach is expected to have an impact outside of cell-based sensing to labs-on-a-chip, microfluidics, and nanotechnology by developing basic technology and techniques for sophisticated manipulation of particles at the micro-scale. The PIs are engineers in several disciplines (fluids and controls, micro-fabrication and conjugated polymers, integrated circuits and biosensors) working closely with cell biologists, molecular pathologists, and experts in bio-functionalized surfaces and quantum dots. The PIs are pioneering the development of MEMS education kits that can be used outside of a clean room.

目标是开发和展示基于细胞的传感技术。细胞诊所是能够捕获和表征细胞的微环境。每个“诊所”都是在CMOS芯片上制造的微机电系统。生物系统具有高特异性、灵敏度和适应性，可以成为高度集成的传感器的一部分。 第一个目标是使用反馈控制、集成电路和微流体技术的工具进行样品制备、细胞加载和系统小型化。研究结果将用于嗅觉传感和低假阳性病原体检测的两项正在进行的工作。 该系统的三个方面将被证明。(1)电渗流控制将从样品中去除所有光学可见（5微米）颗粒。这将去除污垢，灰尘和细菌，并留下气味以呈现给嗅觉细胞传感器。该系统应能够在真实的时间内使用足够高的吞吐量。(2)用于在三维中操纵细胞的介电泳驱动将用于在平面中定位细胞并将它们引导到细胞诊所小瓶中。(3)In为了开发基于现场实用单元的传感器，将开发与用于单元操纵的单元诊所具有相同尺寸的视觉系统。 所提出的技术进步将允许基于细胞的感测走向实际实施并与真实的样本一起使用。基于细胞的传感具有选择性，灵敏度和速度的潜力，远远超过今天的化学和生物传感器。嗅觉传感和病原体检测问题与国家安全直接相关。这项技术在其他不同领域有明显的应用，如医疗保健，药物开发和环境监测。集成的传感-驱动-控制方法预计将在基于细胞的传感之外对芯片实验室、微流体和纳米技术产生影响，方法是开发用于在微观尺度上对颗粒进行复杂操纵的基本技术和技术。PI是多个学科（流体和控制，微制造和共轭聚合物，集成电路和生物传感器）的工程师，与细胞生物学家，分子病理学家以及生物功能化表面和量子点专家密切合作。PI正在率先开发可在洁净室外使用的MEMS教育套件。