SGER: Parallelized Hybrid Solid-State/Bio Microfabrication

该方案的目标是开发一种并行微制造技术，允许将无机材料和微结构与毫米级昆虫集成在一起。具体地说，果蝇被用作模型昆虫。果蝇胚胎通过流体自组装自组装成图案化的底物形成阵列。它们被封装在微腔中，并允许开发周期进行。在幼虫阶段停止发育，以执行微型制造步骤，例如在微室中将金属蒸发到幼虫上，然后允许昆虫发育周期继续进行。这项技术允许以大规模并行的方式修改毫米级的昆虫。在未来的扩展中，它可以用来将控制/射频电路或传感器整合到昆虫群体的结构中。智力优势：该项目开发了一种结合生物学和固态微制造的新型混合制造方法。它提供了第一个在活的和有功能的昆虫上直接微细加工的例子。该项目将确定在昆虫发育的不同水平对其进行并行固态处理的可能性和局限性，并将展示一种大规模并行方法来修改大量生物实体。广泛影响：该项目直接有助于培训学生在生物学和固态制造之间的接口。

The objective of this proposal is to develop a parallel microfabrication technology that allows for integration of inorganic materials and microstructures with mm-scale insects.Specifically, fruit fly is used as a model insect. Fruit fly embryos are self-assembled into a patterned substrate via fluidic self-assembly to form an array. They are encapsulated inmicrochambers and the development cycle is allowed to progress. The development is halted in the larva stage to perform a microfabrication step, for example metal evaporation, onto the larvae in the microchambers and then the insect development cycle is allowed to proceed. This technology allows for modification of mm-scale insects in a massively parallel fashion. In future extensions it can be used to incorporate control/RF circuitry or sensors into the structure of colonies of insects. Intellectual Merits:This project develops a novel hybrid manufacturing method that combines biology and solid-state microfabrication. It provides the first example of direct microfabrication on a live and functional insect. The project will establish the possibilities and limits of parallel solid-state processing on insects at various levels of their development and will demonstrate a massively parallel method to modify a large collection of biological entities.Broader Impact:The project directly contributes to training of students at the interface between biology and solid-state fabrication.