SGER: Nanoporous Conductive Sol-Gel Biocatalysis for Novel Cofactor Regeneration

建议标题：SGER：纳米多孔导电溶胶-凝胶生物催化用于新型辅因子再生建议编号：CTS-0538633主要研究人员：Chan beum Park Institution：Arizona State University分析(决定的基本原理)：这项建议将解决氧化还原酶中辅因子的关键使用，如昂贵的吡啶辅助因子，必须为各种技术应用经济地再生。虽然电化学回收辅因子被认为是一种潜在的有用的方法，但有一个明显的障碍，因为只有电极表面附近的酶才能有效地参与其中。这一探索性研究项目将检验一种高度创新的方法，该方法使用“电子导电”的溶胶-凝胶生物反应器进行辅因子再生。通过将氧化还原酶包埋在导电的溶胶-凝胶杂化基质中，并通过操纵纳米孔凝胶的化学性质，可以假设辅助因子将在整个反应器系统中有效地循环使用。首席研究人员的这一方法显示了对再生过程中关键挑战的敏锐洞察，但该项目显然是高风险的，因为需要制备用于溶胶-凝胶合成的导电溶胶部分/聚吡咯/石墨/金属颗粒的精确“复合材料”，该复合材料将基于硅基溶胶前体，如三烷氧基硅烷。凝胶前体的化学组成必须与原位辅因子再生效率和所需产品产量的知识密切相关。然而，这种方法的成功示范可能会彻底改变电化学再生对生物催化剂的适用性。氧化还原酶辅因子再生的困难和费用是重要附加值产品更广泛合成的关键障碍，包括药品、食品添加剂、香水、杀虫剂和杀虫剂。这种方法将为科学研究开辟一条全新的途径，并将为基础研究和工程带来新的领域。生物传感器和生物燃料电池领域的更广泛的技术应用也是可能的。这项研究的结果将被纳入高级化学工程课程，这将是对加强工程课程的重大贡献。

AbstractProposal Title: SGER: Nanporous Conductive Sol-Gel Biocatalysis for Novel Cofactor RegenerationProposal Number: CTS-0538633Principal Investigator: Chan Beum ParkInstitution: Arizona State UniversityAnalysis (rationale for decision):This proposal will address the critical use of cofactors for oxidoreductases, such as the expensive pyridine cofactor, which must be economically regenerated for a variety of technological applications. Although electrochemical recycling of cofactors has been regarded as a potentially useful approach, there is a clear obstacle in that only enzymes in the immediate vicinity of electrode surface are effectively involved. This exploratory research project will examine a highly innovative approach that uses an "electronically-conductive" sol-gel bioreactor for cofactor regeneration. By entrapping oxidoreductases in a conductive sol-gel hybrid matrix and by manipulating the chemical properties of the nanoporous gel, it is hypothesized that the cofactor will be efficiently recycled throughout the entire reactor system. This approach by the principle investigator shows acute insight into the key challenges in the regeneration process, but the project is clearly high risk because of the necessity of preparing a precise "composite" of conductive sol moiety/polypyrrole/graphite/metal particles for the sol-gel synthesis which would be based on silica-based sol precursors, such as trialkoxysilanes. The chemical composition of the gel precursors must be carefully correlated with knowledge of the in situ cofactor regeneration efficiency and the desired product yield. Successful demonstration of this approach, however, would likely revolutionize the applicability of electrochemical regeneration for biocatalysts. The difficulty and expense of cofactor regeneration for oxidoreductases is a key roadblock for the wider syntheses of important valued-added products including pharmaceuticals, food additives, perfumes, insecticides, and pesticides. This approach would open an entirely new avenue of scientific investigation and would lead to new areas for fundamental research and engineering. Broader technological applications in the areas of biosensors and biofuel cells are also possible. Results from the research will be incorporated into upper-level chemical engineering coursework, which will be a significant contribution to the enhancement of the engineering curriculum.