Osmosensory signal transduction in gill cells of euryhaline tilapia

广盐罗非鱼鳃细胞的渗透感应信号转导

• 批准号: 0542755
• 负责人: Dietmar Kültz
• 金额: $ 52.07万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0542755&HistoricalAwards=false
• 关键词: Osmosensory; signal; transduction; gill; cells

The overall objective of this project is to investigate molecular mechanisms that enable a select group of fishes, including tilapia, to live in a wide range of salinity, including seawater and fresh water. These fishes are called euryhaline and, unlike most fishes, they possess adaptive mechanisms that allow them to tolerate extreme salinity stress and to actively counteract such stress. Using tilapia as a model this project aims to determine the molecular signaling pathways that control salinity adaptation in euryhaline fishes. Proteomics methods (2D electrophoresis, mass spectrometry, bioinformatics), tissue microarray/ laser scanning cytometry analysis, co-immunoprecipitation, and recombinant DNA methodology will be used to address three specific aims: 1. Investigation of the mechanisms by which OSTF1 and 14 3-3 signaling proteins control salinity adaptation; 2. Identification of the signal (plasma cortisol, plasma osmolality, medium salinity) that triggers adaptations mediated by OSTF1 and 14-3-3 during salinity stress; 3. Determination of full-length coding sequence and in-depth characterization of 15 novel salinity-induced tilapia genes recently identified in the PI's laboratory. The overall significance of this work lies in its inherent potential for unraveling major mechanisms of cellular signal transduction that control salinity adaptation and environmental stress tolerance in euryhaline fishes. Knowledge about these mechanisms is critical for understanding why some fish species tolerate environmental stress very well whereas other species are vulnerable to even minor environmental change. The advancement of scientific knowledge by the study of environmental regulation of cell and organismal function has the benefit of providing the tools for predicting, monitoring, and counteracting negative effects of environmental stress on organisms. Thus, the results of this project should have broad applicability to the general cell biology of stress and environmental adaptation. Tilapia are ideal for the proposed studies because this species is important for aquaculture in the US and worldwide. Moreover, tilapia are important from an ecological perspective as they are an invasive species in the US because of their high environmental stress tolerance and adaptability. Important broader impacts of this project also pertain to in-depth training of a postdoctoral researcher and students, including underrepresented minority students. Furthermore, a variety of research, educational, and outreach activities will be supported by this project.

该项目的总体目标是研究使包括罗非鱼在内的一组选定鱼类能够在包括海水和淡水在内的各种盐度下生活的分子机制。这些鱼类被称为广盐性鱼类，与大多数鱼类不同，它们具有适应机制，使它们能够耐受极端的盐度压力，并积极对抗这种压力。以罗非鱼为模型，本项目旨在确定控制广盐性鱼类盐度适应的分子信号通路。蛋白质组学方法（2D电泳，质谱，生物信息学），组织微阵列/激光扫描细胞仪分析，免疫共沉淀和重组DNA方法将用于解决三个具体目标：1。OSTF 1和14个3-3信号蛋白调控盐度适应的机制研究; 2.鉴定在盐胁迫期间触发由OSTF 1和14-3-3介导的适应的信号（血浆皮质醇、血浆渗透压、中等盐度）; 3. PI实验室最近鉴定的15个新的盐度诱导罗非鱼基因的全长编码序列的确定和深入表征。这项工作的整体意义在于其内在的潜力，解开控制广盐性鱼类的盐度适应和环境胁迫耐受性的细胞信号转导的主要机制。了解这些机制对于理解为什么有些鱼类能够很好地耐受环境压力，而其他物种则容易受到即使是微小的环境变化的影响至关重要。通过研究环境对细胞和生物体功能的调节，科学知识的进步有利于为预测、监测和抵消环境压力对生物体的负面影响提供工具。因此，该项目的结果应该对应激和环境适应的一般细胞生物学具有广泛的适用性。罗非鱼是拟议研究的理想选择，因为该物种对美国和全球的水产养殖很重要。此外，从生态角度来看，罗非鱼很重要，因为它们是美国的入侵物种，因为它们具有很高的环境胁迫耐受性和适应性。该项目的重要影响还涉及对博士后研究人员和学生，包括代表性不足的少数民族学生的深入培训。此外，该项目还将支持各种研究、教育和外联活动。