Functional and evolutionary analysis of chloroplast metabolite transporters in the C4 plant maize

C4植物玉米叶绿体代谢转运蛋白的功能和进化分析

• 批准号: 0548610
• 负责人: Susanne Hoffmann-Benning
• 金额: $ 45万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0548610&HistoricalAwards=false
• 关键词: Functional; evolutionary; analysis; chloroplast; metabolite

Due to a biochemical inorganic carbon-concentrating mechanism, C4-photosynthesis is advantageous to C3-photosynthesis under conditions where photosynthesis becomes carbon dioxide-limited, such as high temperature, drought, and saline conditions. The enzymatic steps involved in the C4 inorganic carbon pump have been identified, and the enzymes and genes and their regulation have been characterized in great detail. However, most of the metabolite transporters involved in this biochemical inorganic carbon pump, in particular those of the chloroplast envelope membrane, are unknown. The hypothesis to be tested in this award is that plastid envelope membrane transporters involved in the C4-photosynthetic pathway can be identified based on their abundance in C4 chloroplast envelope membranes and their temporal, developmental, and spatial expression pattern. The investigator proposes to use comparative and quantitative proteomics and transcript profiling to identify candidate genes for these transporters, and reconstitution of purified recombinant transporter proteins into liposomes to functionally characterize the corresponding proteins.These studies are expected to significantly increase understanding of the fundamental physiological process of C4 photosynthesis. The project will lead to in-depth understanding of how a complex biochemical pathway is connected and coordinated by transport proteins in the plastid envelope membrane.Broader impacts resulting from the proposed activityUndergraduate and graduate students will participate in the project. They will receive cross disciplinary training so they can think beyond their individual field of expertise towards an integrative view of plant biology. Research activities will be integrated into teaching by training junior scientists in the handling of membrane transporters, a field currently not represented at this institution. Graduate students and senior researchers will be involved in undergraduate teaching in the field of plant membrane transport. Plant material generated in this project will be used to introduce K-12 teachers into genetic engineering of plants and to train K-12 teachers in modern molecular techniques in summer courses. A public project database will provide the plant research community with information on maize chloroplast envelope membrane proteome.

由于生物化学无机碳浓缩机制，在光合作用变得二氧化碳受限的条件下，例如高温、干旱和盐条件下，C4-光合作用有利于C3-光合作用。 参与C4无机碳泵的酶促步骤已被确定，酶和基因及其调控已被非常详细地表征。 然而，大多数参与这种生化无机碳泵的代谢物转运蛋白，特别是叶绿体被膜的转运蛋白，是未知的。 在这个奖项中要测试的假设是，质体包膜膜转运蛋白参与C4光合途径，可以确定基于其丰富的C4叶绿体包膜膜和他们的时间，发育和空间表达模式。 本研究拟利用比较蛋白质组学、定量蛋白质组学和转录谱分析等方法来鉴定这些转运蛋白的候选基因，并将纯化的重组转运蛋白重组到脂质体中，以表征相应蛋白的功能，这些研究有望显著提高对C4光合作用基本生理过程的认识。 该项目将导致深入了解一个复杂的生化途径是如何连接和协调的运输蛋白质在质体包膜膜。更广泛的影响所产生的拟议活动本科生和研究生将参与该项目。 他们将接受跨学科的培训，使他们能够超越自己的专业领域，对植物生物学的综合观点。 研究活动将通过培训初级科学家处理膜转运蛋白而纳入教学，这是该机构目前没有代表的领域。 研究生和高级研究人员将参与植物膜运输领域的本科教学。 该项目产生的植物材料将用于向K-12教师介绍植物基因工程，并在夏季课程中培训K-12教师掌握现代分子技术。 一个公共项目数据库将为植物研究界提供玉米叶绿体被膜蛋白质组的信息。