NER: A novel Lab-on-Tip nanomechanical platform for single molecule DNA sequencing

NER：一种用于单分子 DNA 测序的新型 Lab-on-Tip 纳米机械平台

• 批准号: 0608835
• 负责人: Yong Xu
• 金额: --
• 依托单位: Wayne State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0608835&HistoricalAwards=false
• 关键词: NER; novel; Lab; Tip; nanomechanical

NER: A novel Lab-on-Tip nanomechanical platform for single molecule DNA sequencing This proposal was received in response to Nanoscale Science and Engineering initiative, NSF 05-610, category NER. The objective of this research is to develop a novel Lab-on-Tip nanomechanical platform with an integrated nanoscale pore for single molecule DNA sequencing. The approach is based on the different unbinding forces between DNA nucleotides. As the target single stranded DNA molecule passes through the nanoscale pore, different pico-Newton level forces will be generated. This force is then detected by the Lab-on-Tip device, which is developed using state-of-the-art micro- and nano-fabrication techniques. Therefore, the DNA sequence can be obtained by monitoring the forces as the single stranded DNA molecule traverses the nanoscale pore. The proposed research has the potential to realize a revolutionary new DNA sequencing method and be a leader in the race to sequence whole human genome for $100,000 in five years, and for $1,000 in a decade. Therefore, this high risk/high return research is likely to bring significant societal benefits because of the crucial roles played by DNA sequencing in various biomedical researches and practical applications. The research will be effectively integrated with education through interdisciplinary courses. The PI is also committed to broaden the participation of underrepresented groups.

NER：一种用于单分子DNA测序的新型Lab-on-Tip纳米机械平台这项建议是对NSF 05-610类纳米科学与工程倡议的响应。本研究的目的是开发一种新型的具有集成纳米孔的Lab-on-Tip纳米机械平台，用于单分子DNA测序。这种方法是基于DNA核苷酸之间不同的解结力。当目标单链DNA分子通过纳米尺度的孔洞时，将产生不同的皮牛顿水平力。这种力随后被尖端实验室设备检测到，该设备是使用最先进的微米和纳米制造技术开发的。因此，当单链DNA分子穿过纳米尺度的孔时，可以通过监测作用力来获得DNA序列。这项拟议中的研究有可能实现一种革命性的新DNA测序方法，并在五年内耗资10万美元、十年耗资1000美元对整个人类基因组进行测序的竞赛中处于领先地位。因此，由于DNA测序在各种生物医学研究和实际应用中所起的关键作用，这种高风险/高回报的研究可能会带来显著的社会效益。研究将通过跨学科课程有效地与教育相结合。国际和平协会还致力于扩大代表性不足群体的参与。