Arabidopsis 2010: Analysis of the Two-component Signaling Network

拟南芥 2010：二元信号网络分析

• 批准号: 0618286
• 负责人: Joseph Kieber
• 金额: --
• 依托单位: University of North Carolina at Chapel Hill
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0618286&HistoricalAwards=false
• 关键词: Arabidopsis; 2010; Analysis; Two; component

The gene network: This project focuses on a gene network that regulates plant responses tocytokinin, a key hormonal regulator of plant growth and development. The initial steps incytokinin signaling are mediated by a 'two-component' system that transmits information frommembrane to nucleus. The two-component system makes use of histidine kinases that act ascytokinin receptors, histidine-containing phosphotransfer proteins that transduce the initialsignal, and response regulators that regulate the signal output. The transcriptional targets of thispathway as well as other outputs have yet to be determined. This project will delineate thenetwork of genes and proteins regulated by two-component signaling elements of Arabidopsis.This two-component signaling network includes the transcription factors that control geneexpression as well as interacting proteins that regulate other aspects of signal output.Project URL: http://www.bio.unc.edu/research/two-component/default.htmFunctional characterization: Transcription factors will be characterized in terms of the genes thatthey regulate. Proteins will be characterized in terms of their protein-protein interactions withinthe signaling network. The physiological function of the network elements will be defined interms of their role in regulating cytokinin responses as well as seed size, meristem function,vascular development, leaf and cotyledon morphology, metal ion homeostasis, and circadianrhythms.Sharing of results: Genomic information and tools generated will be made available to theresearch community through the web and through standard methods of publication in a timelyfashion. Datasets of general interest, that are not made available through publication, will bedeposited on the project web site within two years of their generation.Significance of work: These studies will define the circuitry by which the two-component genenetwork acts to alter plant growth and development. In addition, these studies will revealconnections between this pathway and other cellular networks. Determining how this integrationis achieved will contribute to an understanding of signaling networks in plants and will serve as amodel for examining other interactions within the plant cell. The techniques and tools developedduring the course of these studies will assist gene studies in Arabidopsis and in other plantspecies.Broader impact: Results from the project will benefit society through the understanding of acritical gene network that regulates multiple traits of agronomic importance. Elucidation of theseroles will provide avenues to modify such agriculturally relevant traits such as senescence, planttransformation, grain yield and filling, and patterns of growth and development. The plantresearch community will benefit by the optimization of novel research techniques and thedevelopment of new community resources. The proposed research will enhance theinfrastructure of research and education by providing hands-on training for undergraduatestudents, graduate students, and post-doctoral researchers within the PIs' labs. In addition, thePIs will partner will local groups to assist in the creation and maintenance of programs aimed atfostering science education in grades K-12.

基因网络：该项目的重点是调节植物对细胞分裂素反应的基因网络，细胞分裂素是植物生长和发育的关键激素调节剂。细胞收缩素信号传导的初始步骤是由一个“双组分”系统介导的，该系统将信息从细胞膜传递到细胞核。双组分系统利用了作为细胞分裂素受体的组氨酸激酶、抑制初始信号的含组氨酸磷酸转移蛋白和调节信号输出的反应调节剂。该途径的转录靶点以及其他产物尚未确定。本项目将描述由拟南芥双组分信号元件调控的基因和蛋白质网络.该双组分信号网络包括控制基因表达的转录因子以及调节信号输出其他方面的相互作用蛋白.项目URL：http://www.bio.unc.edu/research/two-component/default.htmFunctional表征：转录因子将根据它们调控的基因来表征.蛋白质将根据其在信号网络中的蛋白质-蛋白质相互作用来表征。网络元素的生理功能将根据它们在调节细胞分裂素反应以及种子大小、分生组织功能、维管发育、叶和子叶形态、金属离子稳态和昼夜节律中的作用来定义。结果共享：基因组信息和生成的工具将通过网络和及时出版的标准方法提供给研究界。一般感兴趣的数据集，没有通过出版物提供，将存放在项目网站在他们generation.Significance工作的两年内：这些研究将确定电路的双组分基因网络的行为，以改变植物的生长和发育。此外，这些研究将揭示该通路与其他细胞网络之间的联系。确定这种整合是如何实现的将有助于理解植物中的信号网络，并将作为研究植物细胞内其他相互作用的模型。在这些研究过程中开发的技术和工具将有助于拟南芥和其他植物物种的基因研究。更广泛的影响：该项目的结果将通过了解调节多种农艺学重要性状的关键基因网络而造福社会。这些性状的阐明将为改变诸如衰老、植物转化、籽粒产量和灌浆以及生长发育模式等农业相关性状提供途径。植物研究社区将受益于新的研究技术的优化和新的社区资源的开发。拟议的研究将通过为PI实验室内的本科生、研究生和博士后研究人员提供实践培训，加强研究和教育的基础设施。此外，PI将与当地团体合作，协助创建和维护旨在促进K-12年级科学教育的计划。