Arabidopsis 2010: Analysis of the Cytokinin-Regulated Transcriptional Network

细胞分裂素是影响多种植物生长发育过程的植物激素。细胞分裂素如何介导如此多样化的过程仍然是植物生物学中具有重要农业意义的基本问题。在这个项目中，遗传学、分子学和基因组学的方法将被用来识别和表征细胞分裂素对基因表达的控制的关键因素。然后，计算方法将用于构建细胞分裂素调节基因表达的网络。这些数据将被纳入一个通过项目网站（http://www.bio.unc.edu/research/two-component/default.htm）提供的数据库中，以便研究界可以很容易地查看和利用监管网络。拟议研究的结果将通过阐明调控多种农艺重要性状的关键基因网络而造福社会。先前的工作表明，该项目的结果将为修改农业相关性状（如粮食产量、能量捕获和利用、衰老、对环境压力的反应以及生长和发育模式）提供途径。该研究将通过为本科生、研究生和博士后研究人员提供培训，以及创建和维持旨在促进K-12年级科学教育的项目，来加强研究和教育的基础设施。

Cytokinins are plant hormones that affect a diverse array of plant growth and development processes. How cytokinins mediate such a diverse array of processes remains a fundamental question in plant biology with important agricultural implications. In this project, genetic, molecular, and genomic approaches will be used to identify and characterize the critical factors that control gene expression in response to cytokinin. Computational approaches will then be used to build a network of cytokinin-regulated gene expression. These data will be incorporated into a database available through the project website (http://www.bio.unc.edu/research/two-component/default.htm) so that the research community can easily view and make use of the regulatory network. Results from the proposed research will benefit society through the elucidation of a critical gene network that regulates multiple traits of agronomic importance. Previous work suggests that results from the project will provide avenues to modify agriculturally relevant traits such as grain yield, energy capture and use, senescence, responses to environmental stresses, and patterns of growth and development. The proposed research will enhance the infrastructure of research and education by providing training for undergraduate students, graduate students, and post-doctoral researchers, as well as the creation and maintenance of programs aimed at fostering science education in grades K-12.