The Regulation of Ethylene Biosynthesis

乙烯生物合成的调控

• 批准号: 1021704
• 负责人: Joseph Kieber
• 金额: $ 50.5万
• 依托单位: University of North Carolina at Chapel Hill
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1021704&HistoricalAwards=false
• 关键词: Regulation; Ethylene; Biosynthesis

Intellectual merit:The gaseous phytohormone ethylene has profound effects on plant growth and development. The biosynthesis of this hormone is influenced by many other hormonal, developmental, and environmental signals, and as such acts as an important point of crosstalk in regulating plant growth and development. This project will explore the mechanisms regulating ethylene biosynthesis in the model plant Arabidopsis thaliana. Previous studies have shown that the regulation of the stability of ACC synthase, a key enzyme in the production of ethylene, is the critical factor controlling how much ethylene plants make. The research supported by this award will build on these findings by charactering the half-lives of different ACC synthase proteins from Arabidopsis to determine how rapidly each is turned-over and how pairing different isoforms together affects their turnover. How other hormones interact to regulate the stability of these different ACS proteins will also be studied. This will help understand how these various signaling pathways influence the production of ethylene. A final aim is to examine the role of proteins that were previously identified as interacting with ACC synthase, with a focus on how these proteins may further regulate the function of ACC synthase. Broader impacts:Results from this research will lead to a deeper understanding of the regulation of ethylene biosynthesis, which may lead to the ability to manipulate the production of this hormone in agricultural settings and hence an improvement in the quality and longevity of various agricultural products. Furthermore, the regulation of protein turnover has emerged as a central mechanism underlying a variety of biological process, and this project will shed light on this fundamental process. These studies will enhance the infrastructure of research and education by providing hands-on training for undergraduate students, graduate students, and post-doctoral researchers. The PI has connections with programs aimed at broadening participation in science. In addition, graduate students are also involved in summer science camp activities for 6-8th graders, providing them hands on science experiments related to plants and ethylene.

智力优势：气体植物激素乙烯对植物生长和发育有深远的影响。这种激素的生物合成受到许多其他激素、发育和环境信号的影响，因此在调节植物生长和发育中充当重要的串扰点。本项目将探讨模式植物拟南芥乙烯生物合成的调控机制。以往的研究表明，ACC合成酶的稳定性的调节，在乙烯的生产中的关键酶，是控制乙烯植物产生多少的关键因素。该奖项支持的研究将建立在这些发现的基础上，通过表征来自拟南芥的不同ACC合酶蛋白的半衰期，以确定每个蛋白的周转速度以及不同亚型的配对如何影响其周转。还将研究其他激素如何相互作用来调节这些不同ACS蛋白的稳定性。这将有助于了解这些不同的信号通路如何影响乙烯的产生。最后一个目的是研究以前被确定为与ACC合酶相互作用的蛋白质的作用，重点是这些蛋白质如何进一步调节ACC合酶的功能。更广泛的影响：这项研究的结果将导致对乙烯生物合成调节的更深入了解，这可能导致在农业环境中操纵这种激素的生产的能力，从而提高各种农产品的质量和寿命。此外，蛋白质周转的调节已经成为各种生物过程的核心机制，本项目将揭示这一基本过程。这些研究将通过为本科生、研究生和博士后研究人员提供实践培训，加强研究和教育的基础设施。PI与旨在扩大科学参与的计划有联系。此外，研究生还参与了6- 8年级学生的暑期科学夏令营活动，为他们提供与植物和乙烯相关的科学实验。