MRI: Development of a 4D micro/nano-scale defocused particle tracking (DPT) imaging technique and its applications in nanobiotechnology

MRI：4D微/纳米级散焦粒子追踪（DPT）成像技术的发展及其在纳米生物技术中的应用

• 批准号: 0619626
• 负责人: Abraham Stroock
• 金额: $ 34万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-15 至 2009-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0619626&HistoricalAwards=false
• 关键词: MRI; Development; 4D; micro; nano

Proposal No. CTS-0619626Principal Investigator: Abraham D. Stroock, Cornell UniversityMRI: Development of a 4-dimensional micro/nano-scale defocused particle tracking (DPT) imaging technique and its applications in nanobiotechnology This Major Research Instrumentation grant is to develop an imaging technology for tracking multiple micro/nano scale particles with sub-micrometer spatial and millisecond temporal resolution via a quantitative use of defocused images taken with an epifluorescence microscope. This new technology is referred to as a 4-dimensional micro/nano defocused particle tracking (4D DPT) technique. The main contribution of the 4D DPT technique to the current state-of-art technology is its capability to probe Lagrangian information of particles at micro and nano scales, which is essential for the development of micro/nano fluidic devices, bioMEMS device, as well as the understanding of inter and intra cellular dynamics in biology. The 4D micro/nano DPT technique represents an important innovation in flow visualization at micro/nano meter scales. The intellectual merit of the proposed project resides in its ability to unravel dynamic behavior both in micro/nano fluidic and biofluidic systems at unprecedented spatial and temporal resolution. When coupled with recent advances in biophotonics, particularly genetically encoded fluorescent reagents, 4D micro/nano DPT technique will enable an ability to visualize single molecular events in living cells. Such capability will, in turn, play a unique role in the recent quest of correlating gene network dynamics with cell's phenotype in biology. In terms of broader impact, the 4D DPT will serve as a shared facility within the Cornell Nanobiotechnology Center (NBTC - NSF STC), it will provide a natural setting for collaboration among its users across many research disciplines. As such, it will impact a broad scientific community. In addition, the 4D DPT technology will be made available to industry through the NBTC's industrial partnership program, and through PIs' collaboration with local industries. The 4D imaging system provides us with a unique opportunity to reach out to the public due to the visual nature of the experimental results. The results of the 4D DPT imaging system will be made into an exhibition entitled `Brownian motion' at the Ithaca Science Center, where visitors can see movie clips of dilute nano and micro particles, as well as swimming bacteria. The PIs will develop an interactive website both for the K-12 community and the research community. For the greater research community, the PIs will establish and maintain an up-to-date website of research results and imaging software.

建议书编号CTS-0619626首席研究员：亚伯拉罕·D·斯特鲁克，康奈尔大学核磁共振成像：四维微/纳米尺度散焦粒子跟踪(DPT)成像技术的开发及其在纳米生物技术中的应用这项重大研究仪器拨款是为了开发一种成像技术，通过定量使用用荧光显微镜拍摄的散焦图像来跟踪具有亚微米空间和毫秒时间分辨率的多个微/纳米尺度粒子。这项新技术被称为四维微/纳米散焦粒子跟踪(4DDPT)技术。四维DPT技术对当前最先进技术的主要贡献是它能够在微米和纳米尺度上探测粒子的拉格朗日信息，这对于开发微/纳米流体器件、生物MEMS器件以及理解生物学中的细胞间和细胞内动力学是必不可少的。微纳四维DPT技术是微纳尺度流动显示领域的一项重大创新。拟议项目的智力优势在于它能够以前所未有的空间和时间分辨率揭示微/纳米流体和生物流体系统中的动态行为。结合生物光子学的最新进展，特别是基因编码的荧光试剂，4D微/纳米DPT技术将使人们能够可视化活细胞中的单分子事件。这种能力反过来将在最近寻求将基因网络动态与生物学中细胞的表型相关联的过程中发挥独特的作用。就更广泛的影响而言，4D DPT将作为康奈尔纳米生物技术中心(NBTC-NSF STC)内的共享设施，它将为其用户之间跨许多研究学科的协作提供一个自然的环境。因此，它将影响广泛的科学界。此外，4D DPT技术将通过NBTC的行业伙伴计划以及PI与当地行业的合作提供给行业。由于实验结果的可视性质，4D成像系统为我们提供了一个接触公众的独特机会。4D DPT成像系统的结果将被制作成伊萨卡科学中心名为《布朗运动》的展览，参观者可以在那里看到稀释的纳米和微米粒子以及游泳细菌的电影剪辑。私人投资机构将为K-12社区和研究社区开发一个互动网站。对于更广泛的研究社区，私人投资促进机构将建立和维护一个最新的研究成果和成像软件网站。